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dc.contributor.authorNyathi, Yvonne
dc.contributor.authorPool, M.R.
dc.date.accessioned2020-06-10T07:46:45Z
dc.date.accessioned2020-07-02T11:12:12Z
dc.date.available2020-06-10T07:46:45Z
dc.date.available2020-07-02T11:12:12Z
dc.date.issued2015-07
dc.identifier.citationNyathi Y and Pool MR (2015) Analysis of the interplay of protein biogenesis factors at the ribosome exit site reveals new role for NAC. Journal of Cell Biology. 210(2): 287-301.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17895
dc.descriptionYesen_US
dc.description.abstractThe ribosome exit site is a focal point for the interaction of protein-biogenesis factors that guide the fate of nascent polypeptides. These factors include chaperones such as NAC, N-terminal-modifying enzymes like Methionine aminopeptidase (MetAP), and the signal recognition particle (SRP), which targets secretory and membrane proteins to the ER. These factors potentially compete with one another in the short time-window when the nascent chain first emerges at the exit site, suggesting a need for regulation. Here, we show that MetAP contacts the ribosome at the universal adaptor site where it is adjacent to the α subunit of NAC. SRP is also known to contact the ribosome at this site. In the absence of NAC, MetAP and SRP antagonize each other, indicating a novel role for NAC in regulating the access of MetAP and SRP to the ribosome. NAC also functions in SRP-dependent targeting and helps to protect substrates from aggregation before translocation.en_US
dc.description.sponsorshipThis work was supported by grants from the BBSRC [H007202/1] and Wellcome Trust [097820/Z/11/A].en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1083/jcb.201410086en_US
dc.rights© 2015 Nyathi and Pool. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).en_US
dc.subjectProtein biogenesisen_US
dc.subjectProtein targetingen_US
dc.subjectProtein translocationen_US
dc.subjectRibosomeen_US
dc.subjectSignal recognition particleen_US
dc.subjectNACen_US
dc.titleAnalysis of the interplay of protein biogenesis factors at the ribosome exit site reveals new role for NACen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-06-03
dc.date.application2015
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2020-06-10T06:46:45Z
refterms.dateFOA2020-07-02T11:13:00Z


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