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dc.contributor.authorLeznicki, P.
dc.contributor.authorKorac-Prlic, J.
dc.contributor.authorKliza, K.
dc.contributor.authorHusnjak, K.
dc.contributor.authorNyathi, Yvonne
dc.contributor.authorDikic, I.
dc.contributor.authorHigh, S.
dc.date.accessioned2020-06-10T07:46:10Z
dc.date.accessioned2020-07-02T10:56:28Z
dc.date.available2020-06-10T07:46:10Z
dc.date.available2020-07-02T10:56:28Z
dc.date.issued2015-09
dc.identifier.citationLeznicki P, Korac-Prlic J, Kliza K et al (2015) Binding of SGTA to Rpn13 selectively modulates protein quality control. Journal of Cell Science. 128(17): 3187-3196.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17894
dc.descriptionYesen_US
dc.description.abstractRpn13 is an intrinsic ubiquitin receptor of the 26S proteasome regulatory subunit that facilitates substrate capture prior to degradation. Here we show that the C-terminal region of Rpn13 binds to the tetratricopeptide repeat (TPR) domain of SGTA, a cytosolic factor implicated in the quality control of mislocalised membrane proteins (MLPs). The overexpression of SGTA results in a substantial increase in steady-state MLP levels, consistent with an effect on proteasomal degradation. However, this effect is strongly dependent upon the interaction of SGTA with the proteasomal component Rpn13. Hence, overexpression of the SGTA-binding region of Rpn13 or point mutations within the SGTA TPR domain both inhibit SGTA binding to the proteasome and substantially reduce MLP levels. These findings suggest that SGTA can regulate the access of MLPs to the proteolytic core of the proteasome, implying that a protein quality control cycle that involves SGTA and the BAG6 complex can operate at the 19S regulatory particle. We speculate that the binding of SGTA to Rpn13 enables specific polypeptides to escape proteasomal degradation and/or selectively modulates substrate degradation.en_US
dc.description.sponsorshipBBSRC [grant number: BB/L006510/1] and the Wellcome Trust [grant number: 092107/Z/10/Z]. K.K. was supported by the UPStream network [EU, FP7, ITN project 290257]en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1242/jcs.165209en_US
dc.rights© 2015. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en_US
dc.subjectBag6en_US
dc.subjectMislocalised proteinsen_US
dc.subjectProteasomesen_US
dc.subjectProtein degradationen_US
dc.subjectTPRen_US
dc.subjectUbiquitylationen_US
dc.titleBinding of SGTA to Rpn13 selectively modulates protein quality controlen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-07-03
dc.date.application2015-07-13
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2020-06-10T06:46:17Z
refterms.dateFOA2020-07-02T10:57:29Z


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