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dc.contributor.authorThapaliya, A.
dc.contributor.authorNyathi, Yvonne
dc.contributor.authorMartínez-Lumbreras, S.
dc.contributor.authorKrysztofinska, E.M.
dc.contributor.authorEvans, N.J.
dc.contributor.authorTerry, I.L.
dc.contributor.authorHigh, S.
dc.contributor.authorIsaacson, R.L.
dc.date.accessioned2020-06-08T01:49:37Z
dc.date.accessioned2020-07-02T08:41:36Z
dc.date.available2020-06-08T01:49:37Z
dc.date.available2020-07-02T08:41:36Z
dc.date.issued2016-11
dc.identifier.citationThapaliya A, Nyathi Y, Martínez-Lumbreras S et al (2016) SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism. Scientific Reports. 6: 36622.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17893
dc.descriptionYesen_US
dc.description.abstractThe fate of secretory and membrane proteins that mislocalize to the cytosol is decided by a collaboration between cochaperone SGTA (small, glutamine-rich, tetratricopeptide repeat protein alpha) and the BAG6 complex, whose operation relies on multiple transient and subtly discriminated interactions with diverse binding partners. These include chaperones, membrane-targeting proteins and ubiquitination enzymes. Recently a direct interaction was discovered between SGTA and the proteasome, mediated by the intrinsic proteasomal ubiquitin receptor Rpn13. Here, we structurally and biophysically characterize this binding and identify a region of the Rpn13 C-terminal domain that is necessary and sufficient to facilitate it. We show that the contact occurs through a carboxylate clamp-mediated molecular recognition event with the TPR domain of SGTA, and provide evidence that the interaction can mediate the association of Rpn13 and SGTA in a cellular context.en_US
dc.description.sponsorshipRLI was supported by MRC New Investigator Research Grant: G0900936. RLI and SH are funded by BBSRC grants: BB/L006952/1 and BB/L006510/1 respectively. RLI is funded by BBSRC grant: BB/N006267/1. AT is funded by BBSRC grant: BB/J014567/1. ILT was the recipient of a Wellcome Trust Vacation Scholarship 2015. NMR experiments were performed at the Centre for Biomolecular Spectroscopy, King’s College London, established with a Capital Award from the Wellcome Trusten_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1038/srep36622en_US
dc.rights© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.subjectSGTAen_US
dc.subjectDiverse binding partnersen_US
dc.subjectProteasomal ubiquitin receptor Rpn13en_US
dc.subjectCarboxylate clamp mechanismen_US
dc.titleSGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanismen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-10-18
dc.date.application2016-11-09
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2020-06-08T00:49:37Z
refterms.dateFOA2020-07-02T08:42:23Z


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