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    SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism

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    Nyathi_Scientific_Reports.pdf (5.148Mb)
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    Publication date
    2016-11
    Author
    Thapaliya, A.
    Nyathi, Yvonne
    Martínez-Lumbreras, S.
    Krysztofinska, E.M.
    Evans, N.J.
    Terry, I.L.
    High, S.
    Isaacson, R.L.
    Keyword
    SGTA
    Diverse binding partners
    Proteasomal ubiquitin receptor Rpn13
    Carboxylate clamp mechanism
    Rights
    © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
    Peer-Reviewed
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    Abstract
    The fate of secretory and membrane proteins that mislocalize to the cytosol is decided by a collaboration between cochaperone SGTA (small, glutamine-rich, tetratricopeptide repeat protein alpha) and the BAG6 complex, whose operation relies on multiple transient and subtly discriminated interactions with diverse binding partners. These include chaperones, membrane-targeting proteins and ubiquitination enzymes. Recently a direct interaction was discovered between SGTA and the proteasome, mediated by the intrinsic proteasomal ubiquitin receptor Rpn13. Here, we structurally and biophysically characterize this binding and identify a region of the Rpn13 C-terminal domain that is necessary and sufficient to facilitate it. We show that the contact occurs through a carboxylate clamp-mediated molecular recognition event with the TPR domain of SGTA, and provide evidence that the interaction can mediate the association of Rpn13 and SGTA in a cellular context.
    URI
    http://hdl.handle.net/10454/17893
    Version
    Published version
    Citation
    Thapaliya A, Nyathi Y, Martínez-Lumbreras S et al (2016) SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism. Scientific Reports. 6: 36622.
    Link to publisher’s version
    https://doi.org/10.1038/srep36622
    Type
    Article
    Collections
    Life Sciences Publications

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