SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism
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2016-11Author
Thapaliya, A.Nyathi, Yvonne
Martínez-Lumbreras, S.
Krysztofinska, E.M.
Evans, N.J.
Terry, I.L.
High, S.
Isaacson, R.L.
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© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/Peer-Reviewed
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The fate of secretory and membrane proteins that mislocalize to the cytosol is decided by a collaboration between cochaperone SGTA (small, glutamine-rich, tetratricopeptide repeat protein alpha) and the BAG6 complex, whose operation relies on multiple transient and subtly discriminated interactions with diverse binding partners. These include chaperones, membrane-targeting proteins and ubiquitination enzymes. Recently a direct interaction was discovered between SGTA and the proteasome, mediated by the intrinsic proteasomal ubiquitin receptor Rpn13. Here, we structurally and biophysically characterize this binding and identify a region of the Rpn13 C-terminal domain that is necessary and sufficient to facilitate it. We show that the contact occurs through a carboxylate clamp-mediated molecular recognition event with the TPR domain of SGTA, and provide evidence that the interaction can mediate the association of Rpn13 and SGTA in a cellular context.Version
Published versionCitation
Thapaliya A, Nyathi Y, Martínez-Lumbreras S et al (2016) SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism. Scientific Reports. 6: 36622.Link to Version of Record
https://doi.org/10.1038/srep36622Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1038/srep36622