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dc.contributor.authorMurat, Katarzyna
dc.contributor.authorGrüning, B.
dc.contributor.authorPoterlowicz, P.W.
dc.contributor.authorWestgate, Gillian E.
dc.contributor.authorTobin, Desmond J.
dc.contributor.authorPoterlowicz, Krzysztof
dc.date.accessioned2020-06-26T11:19:14Z
dc.date.accessioned2020-06-26T14:14:39Z
dc.date.available2020-06-26T11:19:14Z
dc.date.available2020-06-26T14:14:39Z
dc.date.issued2020-05
dc.identifier.citationMurat K, Grüning B, Poterlowicz PW et al (2020) Ewastools: Infinium Human Methylation BeadChip pipeline for population epigenetics integrated into Galaxy. Gigascience. 9(5): giaa049.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17880
dc.descriptionYesen_US
dc.description.abstractInfinium Human Methylation BeadChip is an array platform for complex evaluation of DNA methylation at an individual CpG locus in the human genome based on Illumina’s bead technology and is one of the most common techniques used in epigenome-wide association studies. Finding associations between epigenetic variation and phenotype is a significant challenge in biomedical research. The newest version, HumanMethylationEPIC, quantifies the DNA methylation level of 850,000 CpG sites, while the previous versions, HumanMethylation450 and HumanMethylation27, measured >450,000 and 27,000 loci, respectively. Although a number of bioinformatics tools have been developed to analyse this assay, they require some programming skills and experience in order to be usable. Results We have developed a pipeline for the Galaxy platform for those without experience aimed at DNA methylation analysis using the Infinium Human Methylation BeadChip. Our tool is integrated into Galaxy (http://galaxyproject.org), a web-based platform. This allows users to analyse data from the Infinium Human Methylation BeadChip in the easiest possible way. Conclusions The pipeline provides a group of integrated analytical methods wrapped into an easy-to-use interface. Our tool is available from the Galaxy ToolShed, GitHub repository, and also as a Docker image. The aim of this project is to make Infinium Human Methylation BeadChip analysis more flexible and accessible to everyone.en_US
dc.language.isoenen_US
dc.publisherOxford Academic
dc.relation.isreferencedbyhttps://dx.doi.org/10.1093%2Fgigascience%2Fgiaa049en_US
dc.rights(c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
dc.subjectDNA methylationen_US
dc.subjectEpigenome-wide association studiesen_US
dc.subjectEWASen_US
dc.subjectGalaxy projectsen_US
dc.subjectInfinium Human Mehylationen_US
dc.subjectBeadChipen_US
dc.titleEwastools: Infinium Human Methylation BeadChip pipeline for population epigenetics integrated into Galaxyen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-04-21
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2020-06-26T10:19:24Z
refterms.dateFOA2020-06-26T14:18:27Z


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