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dc.contributor.authorCrompton, R.
dc.contributor.authorWilliams, H.
dc.contributor.authorAnsell, David
dc.contributor.authorCampbell, Laura
dc.contributor.authorHolden, K.
dc.contributor.authorCruickshank, S.
dc.contributor.authorHardman, M.J.
dc.date.accessioned2020-05-06T13:30:44Z
dc.date.accessioned2020-05-14T09:03:03Z
dc.date.available2020-05-06T13:30:44Z
dc.date.available2020-05-14T09:03:03Z
dc.date.issued2016-04
dc.identifier.citationCrompton R, Williams H, Ansell DM, et al (2016) Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair. Laboratory Investigation, 96: 439-449en_US
dc.identifier.urihttp://hdl.handle.net/10454/17812
dc.descriptionnoen_US
dc.description.abstractWound infection is a major clinical problem, yet understanding of bacterial host interactions in the skin remains limited. Microbe-derived molecules, known as pathogen-associated molecular patterns, are recognised in barrier tissues by pattern-recognition receptors. In particular, the pathogen-associated molecular pattern, lipopolysaccharide (LPS), a component of microbial cell walls and a specific ligand for Toll-like receptor 4, has been widely used to mimic systemic and local infection across a range of tissues. Here we administered LPS derived from Klebsiella pneumoniae, a species of bacteria that is emerging as a wound-associated pathogen, to full-thickness cutaneous wounds in C57/BL6 mice. Early in healing, LPS-treated wounds displayed increased local apoptosis and reduced proliferation. Subsequent healing progression was delayed with reduced re-epithelialisation, increased proliferation, a heightened inflammatory response and perturbed wound matrix deposition. Our group and others have previously demonstrated the beneficial effects of 17β-estradiol treatment across a range of preclinical wound models. Here we asked whether oestrogen would effectively promote healing in our LPS bacterial infection model. Intriguingly, co-treatment with 17β-estradiol was able to promote re-epithelialisation, dampen inflammation and induce collagen deposition in our LPS-delayed healing model. Collectively, these studies validate K. pneumoniae-derived LPS treatment as a simple yet effective model of bacterial wound infection, while providing the first indication that oestrogen could promote cutaneous healing in the presence of infection, further strengthening the case for its therapeutic use.en_US
dc.language.isoenen_US
dc.subjectCutaneous woundsen_US
dc.subjectWound repairen_US
dc.subjectWound infectionen_US
dc.subjectBacterial wound infectionen_US
dc.subjectOestrogenen_US
dc.subject17β-estradiol treatmenten_US
dc.subjectSkinen_US
dc.titleOestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repairen_US
dc.status.refereedyesen_US
dc.date.Accepted2015-12-11
dc.typeArticleen_US
dc.type.versionNo full-text in the repositoryen_US
dc.identifier.doihttps://doi.org/10.1038/labinvest.2015.160
dc.date.updated2020-05-06T12:30:46Z


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