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dc.contributor.authorKorzow Richter, K.
dc.contributor.authorMcGrath, K.
dc.contributor.authorMasson-MacLean, E.
dc.contributor.authorHickinbotham, S.
dc.contributor.authorTedder, Andrew
dc.contributor.authorBritton, K.
dc.contributor.authorBottomley, Z.
dc.contributor.authorDobney, K.
dc.contributor.authorHulme-Beaman, A.
dc.contributor.authorZona, M.
dc.contributor.authorFischer, R.
dc.contributor.authorCollins, M.J.
dc.contributor.authorSpeller, C.F.
dc.date.accessioned2020-04-07T13:30:29Z
dc.date.accessioned2020-05-06T07:46:22Z
dc.date.available2020-04-07T13:30:29Z
dc.date.available2020-05-06T07:46:22Z
dc.date.issued2020-04
dc.identifier.citationKorzow Richter K, McGrath K, Masson-MacLean E et al (2020) What's the catch? Archaeological application of rapid collagen-based species identification for Pacific Salmon. Journal of Archaeological Science. 116: 105116.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17771
dc.descriptionYesen_US
dc.description.abstractPacific salmon (Oncorhynchus spp.) are ecological and cultural keystone species along the Northwest Coast of North America and are ubiquitous in archaeological sites of the region. The inability to morphologically identify salmonid post-cranial remains to species, however, can limit our understanding of the ecological and cultural role different taxa played in the seasonal subsistence practices of Indigenous groups in the past. Here, we present a rapid, cost-effective ZooMS method to distinguish salmonid species based on collagen peptide mass-fingerprinting. Using modern reference material and an assemblage of 28 DNA-identified salmonid bones from the pre-contact Yup'ik site of Nunalleq, Western Alaska, we apply high-resolution mass spectrometry (LC-MS/MS) to identify a series of potential collagen peptide markers to distinguish Pacific salmon. We then confirm these peptide markers with a blind ZooMS analysis (MALDI-TOF-MS) of the archaeological remains. We successfully distinguish five species of anadromous salmon with this ZooMS approach, including one specimen that could not be identified through ancient DNA analysis. Our biomolecular identification of chum (43%), sockeye (21%), chinook (18%), coho (11%) and pink (7%), confirm the exploitation of all five available species of salmonid at Nunalleq.en_US
dc.language.isoenen_US
dc.rights© 2020 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.subjectZooMSen_US
dc.subjectCollagen peptideen_US
dc.subjectMass fingerprintingen_US
dc.subjectPacific salmonen_US
dc.subjectYup'Iken_US
dc.subjectNunalleqen_US
dc.titleWhat's the catch? Archaeological application of rapid collagen-based species identification for Pacific Salmonen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-02-19
dc.date.application2020-03-04
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.identifier.doihttps://doi.org/10.1016/j.jas.2020.105116
dc.date.updated2020-04-07T12:30:29Z
refterms.dateFOA2020-05-06T09:28:27Z


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