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dc.contributor.authorAkhtar, Shabana
dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorIsreb, Mohammad
dc.contributor.authorNewton, L.
dc.contributor.authorGopalan, Rajendran C.
dc.contributor.authorAnderson, Diana
dc.date.accessioned2020-05-04T09:39:33Z
dc.date.available2020-05-04T09:39:33Z
dc.date.issued2020-04
dc.identifier.citationAkhtar S, Najafzadeh M, Isreb M et al (2020) ROS-induced oxidative damage in lymphocytes ex vivo/in vitro from healthy individuals and MGUS patients: protection by myricetin bulk and nanoforms. Archives of Toxicology. 94: 1229-1239.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17764
dc.descriptionYesen_US
dc.description.abstractWe investigated the protective role of myricetin bulk and nanoforms, against reactive oxygen species (ROS)-induced oxidative stress caused by hydrogen peroxide and tertiary-butyl hydro peroxide in lymphocytes in vitro from healthy individuals and those from pre-cancerous patients suffering with monoclonal gammopathy of undetermined significance (MGUS). The change in intracellular reactive oxygen species was measured once cells were treated with myricetin bulk forms and nanoforms with and without either hydrogen peroxide or tertiary-butyl hydro peroxide co-supplementation. The direct and indirect antioxidant activity of myricetin was spectrofluometrically measured using the fluorescent dye 2',7'-dichlorofluorescin diacetate and using the Comet assay, respectively. Hydrogen peroxide (50 µM) and tertiary-butyl hydro peroxide (300 µM) induced a higher level of reactive oxygen species-related DNA damage and strand breaks. Addition of myricetin nanoform (20 µM) and bulk (10 µM) form could, however, significantly prevent hydrogen peroxide- and tertiary-butyl hydro peroxide-induced oxidative imbalances and the nanoform was more effective. Glutathione levels were also quantified using a non-fluorescent dye. Results suggest that myricetin treatment had no significant effect on the cellular antioxidant enzyme, glutathione. The current study also investigates the effect of myricetin on the induction of double-strand breaks by staining the gamma-H2AX foci immunocytochemically. It was observed that myricetin does not induce double-strand breaks at basal levels rather demonstrated a protective effect.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1007/s00204-020-02688-4en_US
dc.rights(c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectLymphocytesen_US
dc.subjectMGUS patientsen_US
dc.subjectMyricetin bulk forms and nanoformsen_US
dc.subjectTBHPen_US
dc.subjectHydrogen peroxideen_US
dc.subjectγ-H2AXen_US
dc.titleROS-induced Oxidative Damage in Lymphocytes Ex Vivo/in Vitro From Healthy Individuals and MGUS Patients: Protection by Myricetin Bulk and Nanoformsen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-02-21
dc.date.application2020-02-27
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2020-05-04T09:41:35Z


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