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    The Dictyostelium discoideum RACK1 orthologue has roles in growth and development

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    Swaminathan_CCS_Final.pdf (4.025Mb)
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    Publication date
    2014-06
    Author
    Omosigho, N.N.
    Swaminathan, Karthic
    Plomann, M.
    Müller-Taubenberger, A.
    Noegel, A.A.
    Riyahi, T.Y.
    Keyword
    Dictyostelium discoideum
    G protein signaling
    RACK1
    WD40 repeat protein
    Phosphoinositides
    Phosphorylation
    Dimerization
    Rights
    © 2014 Omosigho et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
    Peer-Reviewed
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    Abstract
    Background: The receptor for activated C-kinase 1 (RACK1) is a conserved protein belonging to the WD40 repeat family of proteins. It folds into a beta propeller with seven blades which allow interactions with many proteins. Thus it can serve as a scaffolding protein and have roles in several cellular processes. Results: We identified the product of the Dictyostelium discoideum gpbB gene as the Dictyostelium RACK1 homolog. The protein is mainly cytosolic but can also associate with cellular membranes. DdRACK1 binds to phosphoinositides (PIPs) in protein-lipid overlay and liposome-binding assays. The basis of this activity resides in a basic region located in the extended loop between blades 6 and 7 as revealed by mutational analysis. Similar to RACK1 proteins from other organisms DdRACK1 interacts with G protein subunits alpha, beta and gamma as shown by yeast two-hybrid, pulldown, and immunoprecipitation assays. Unlike the Saccharomyces cerevisiae and Cryptococcus neoformans RACK1 proteins it does not appear to take over Gβ function in D. discoideum as developmental and other defects were not rescued in Gβ null mutants overexpressing GFP-DdRACK1. Overexpression of GFP-tagged DdRACK1 and a mutant version (DdRACK1mut) which carried a charge-reversal mutation in the basic region in wild type cells led to changes during growth and development. Conclusion: DdRACK1 interacts with heterotrimeric G proteins and can through these interactions impact on processes specifically regulated by these proteins.
    URI
    http://hdl.handle.net/10454/17711
    Version
    Published version
    Citation
    Omosigho NN, Swaminathan K, Plomann M et al (2014) The Dictyostelium discoideum RACK1 orthologue has roles in growth and development. Cell Communication and Signaling. 12: Article number 37.
    Link to publisher’s version
    https://doi.org/10.1186/1478-811X-12-37
    Type
    Article
    Collections
    Life Sciences Publications

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