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    A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin

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    Swaminathan_PNAS.pdf (3.719Mb)
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    Publication date
    2014-01
    Author
    Swaminathan, Karthic
    Müller-Taubenberger, A.
    Faix, J.
    Rivero, F.
    Noegel, A.A.
    Keyword
    Rac GTPases
    Protein-protein interaction
    Cytoskeleton
    Cell signalling
    Rights
    © 2013 The Authors. Reproduced in accordance with the publisher's self-archiving policy.
    Peer-Reviewed
    Yes
    
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    Abstract
    The Cdc42- and Rac-interactive binding motif (CRIB) of coronin binds to Rho GTPases with a preference for GDP-loaded Rac. Mutation of the Cdc42- and Rac-interactive binding motif abrogates Rac binding. This results in increased 1evels of activated Rac in coronin-deficient Dictyostelium cells (corA−), which impacts myosin II assembly. corA− cells show increased accumulation of myosin II in the cortex of growth-phase cells. Myosin II assembly is regulated by myosin heavy chain kinase–mediated phosphorylation of its tail. Kinase activity depends on the activation state of the p21-activated kinase a. The myosin II defect of corA− mutant is alleviated by dominant-negative p21-activated kinase a. It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and Rac-interactive binding motif failed to rescue the myosin defect in corA− mutant cells. Ectopically expressed myosin heavy chain kinases affinity purified from corA− cells show reduced kinase activity. We propose that coronin through its affinity for GDP–Rac regulates the availability of GTP–Rac for activation of downstream effectors.
    URI
    http://hdl.handle.net/10454/17710
    Version
    Accepted manuscript
    Citation
    Swaminathan K, Müller-Taubenberger A, Faix J et al (2014) A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin. PNAS: Proceedings of the National Academy of Sciences of the United States of America. 111(1): E25-E33.
    Link to publisher’s version
    https://doi.org/10.1073/pnas.1315368111
    Type
    Article
    Collections
    Life Sciences Publications

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