Time-resolved crystallography using the Hadamard transform
dc.contributor.author | Yorke, Briony A. | |
dc.contributor.author | Beddard, G.S. | |
dc.contributor.author | Owen, R.L. | |
dc.contributor.author | Pearson, A.R. | |
dc.date.accessioned | 2020-01-22T10:25:28Z | |
dc.date.available | 2020-01-22T10:25:28Z | |
dc.date.issued | 2014-11 | |
dc.identifier.citation | Yorke BA, Beddard GS, Owen RL et al (2014) Time-resolved crystallography using the Hadamard transform. Nature Methods. 11: 1131-1134. | |
dc.identifier.uri | http://hdl.handle.net/10454/17592 | |
dc.description | Yes | |
dc.description.abstract | We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy. | |
dc.description.sponsorship | Wellcome Trust 4-year PhD program “The Molecular Basis of Biological Mechanisms” 089312/Z/09/Z. This work was also supported by the EPSRC Award “Dynamic Structural Science at the Research Complex at Harwell” EP/I01974X/1 and by BBSRC Award BB/H001905/1. | |
dc.language.iso | en | |
dc.rights | © 2014, Springer Nature. Reproduced in accordance with the publisher's self-archiving policy. The final publication is available at https://doi.org/10.1038/nmeth.3139. | |
dc.subject | Data acquisition | |
dc.subject | Molecular biophysics | |
dc.subject | Proteins | |
dc.subject | X-ray crystallography | |
dc.title | Time-resolved crystallography using the Hadamard transform | |
dc.status.refereed | Yes | |
dc.date.application | 05/10/2014 | |
dc.type | Article | |
dc.type.version | Accepted manuscript | |
dc.identifier.doi | https://doi.org/10.1038/nmeth.3139 | |
dc.rights.license | Unspecified | |
refterms.dateFOA | 2020-01-22T10:25:56Z | |
dc.openaccess.status | openAccess | |
dc.date.accepted | 04/09/2014 |