Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis
View/ Open
eLife.pdf (3.485Mb)
Download
Publication date
2016-11-18Keyword
Holliday junction resolvaseS. cerevisiae
VMA1-derived endonuclease
Chromosome structure
Chromosomes
Genes
Homologous recombination mechanisms
Meiotic chromosome axis
Rights
CC0. This is a Public Domain article free of copyright.Peer-Reviewed
YesOpen Access status
openAccess
Metadata
Show full item recordAbstract
Abstract The budding yeast genome contains regions where meiotic recombination initiates more frequently than in others. This pattern parallels enrichment for the meiotic chromosome axis proteins Hop1 and Red1. These proteins are important for Spo11-catalyzed double strand break formation; their contribution to crossover recombination remains undefined. Using the sequence-specific VMA1-derived endonuclease (VDE) to initiate recombination in meiosis, we show that chromosome structure influences the choice of proteins that resolve recombination intermediates to form crossovers. At a Hop1-enriched locus, most VDE-initiated crossovers, like most Spo11-initiated crossovers, required the meiosis-specific MutLγ resolvase. In contrast, at a locus with lower Hop1 occupancy, most VDE-initiated crossovers were MutLγ-independent. In pch2 mutants, the two loci displayed similar Hop1 occupancy levels, and VDE-induced crossovers were similarly MutLγ-dependent. We suggest that meiotic and mitotic recombination pathways coexist within meiotic cells, and that features of meiotic chromosome structure determine whether one or the other predominates in different regions.Version
Published versionCitation
Medhi D, Goldman ASH and Licthen M (2016) Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis. eLife. 5: e19669.Link to Version of Record
https://doi.org/10.7554/eLife.19669Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.7554/eLife.19669