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    Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis

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    eLife.pdf (3.485Mb)
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    Publication date
    2016-11-18
    Author
    Medhi, D.
    Goldman, Alastair S.H.
    Lichten, M.
    Keyword
    Holliday junction resolvase
    S. cerevisiae
    VMA1-derived endonuclease
    Chromosome structure
    Chromosomes
    Genes
    Homologous recombination mechanisms
    Meiotic chromosome axis
    Rights
    CC0. This is a Public Domain article free of copyright.
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Abstract The budding yeast genome contains regions where meiotic recombination initiates more frequently than in others. This pattern parallels enrichment for the meiotic chromosome axis proteins Hop1 and Red1. These proteins are important for Spo11-catalyzed double strand break formation; their contribution to crossover recombination remains undefined. Using the sequence-specific VMA1-derived endonuclease (VDE) to initiate recombination in meiosis, we show that chromosome structure influences the choice of proteins that resolve recombination intermediates to form crossovers. At a Hop1-enriched locus, most VDE-initiated crossovers, like most Spo11-initiated crossovers, required the meiosis-specific MutLγ resolvase. In contrast, at a locus with lower Hop1 occupancy, most VDE-initiated crossovers were MutLγ-independent. In pch2 mutants, the two loci displayed similar Hop1 occupancy levels, and VDE-induced crossovers were similarly MutLγ-dependent. We suggest that meiotic and mitotic recombination pathways coexist within meiotic cells, and that features of meiotic chromosome structure determine whether one or the other predominates in different regions.
    URI
    http://hdl.handle.net/10454/17330
    Version
    Published version
    Citation
    Medhi D, Goldman ASH and Licthen M (2016) Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis. eLife. 5: e19669.
    Link to publisher’s version
    https://doi.org/10.7554/eLife.19669
    Type
    Article
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    Life Sciences Publications

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