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dc.contributor.authorTadesse, M.
dc.contributor.authorAbebe, G.
dc.contributor.authorBekele, A.
dc.contributor.authorBezabih, M.
dc.contributor.authorde Rijk, P.
dc.contributor.authorMeehan, Conor J.
dc.contributor.authorde Jong, B.C.
dc.contributor.authorRigouts, L.
dc.date.accessioned2019-09-24T10:46:05Z
dc.date.accessioned2019-10-17T13:58:52Z
dc.date.available2019-09-24T10:46:05Z
dc.date.available2019-10-17T13:58:52Z
dc.date.issued2017-11
dc.identifier.citationTadesse M, Abebe G, Bekele A et al (2017) The predominance of Ethiopian specific Mycobacterium tuberculosis families and minimal contribution of Mycobacterium bovis in tuberculous lymphadenitis patients in Southwest Ethiopia. Infection, Genetics and Evolution. 55: 251-259.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17305
dc.descriptionNoen_US
dc.description.abstractBackground: Ethiopia has an extremely high rate of extrapulmonary tuberculosis, dominated by tuberculous lymphadenitis (TBLN). However, little is known about Mycobacterium tuberculosis complex (MTBc) lineages re-sponsible for TBLN in Southwest Ethiopia.Methods:A total of 304 MTBc isolates from TBLN patients in Southwest Ethiopia were genotyped primarily by spoligotyping. Isolates of selected spoligotypes were further analyzed by 15-loci mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (n = 167) and qPCR-based single nucleotide polymorphism (n = 38). Isolates were classified into main phylogenetic lineages and families by using the re-ference strain collections and identification tools available at MIRU-VNTRplus data base. Resistance to rifampicin was determined by Xpert MTB/RIF. Results: The majority of isolates (248; 81.6%) belonged to the Euro-American lineage (Lineage 4), with the ill-defined T and Haarlem as largest families comprising 116 (38.2%) and 43 (14.1%) isolates respectively. Of the T family, 108 isolates were classified as being part of the newly described Ethiopian families, namely Ethiopia_2(n = 44), Ethiopia_3 (n = 34) and Ethiopia_H37Rv-like (n = 30). Other sub-lineages included URAL (n = 18), S(n = 17), Uganda I (n = 16), LAM (n = 13), X (n = 5), TUR (n = 5), Uganda II (n = 4) and unknown (n = 19).Lineage 3 (Delhi/CAS) was the second most common lineage comprising 44 (14.5%) isolates. Interestingly, six isolates (2%) were belonged to Lineage 7, unique to Ethiopia. Lineage 1 (East-African Indian) and Lineage 2(Beijing) were represented by 3 and 1 isolates respectively.M. bovis was identified in only two (0.7%) TBLN cases. The cluster rate was highest for Ethiopia_3 isolates showing clonal similarity with isolates from North Ethiopia. Lineage 3 was significantly associated with rifampicin resistance. Conclusions: In TBLN in Southwest Ethiopia, the recently described Ethiopia specific Lineage 4 families were predominant, followed by Lineage 3 and Lineage 4-Haarlem. The contribution of M. bovis in TBLN infection is minimal.en_US
dc.description.sponsorshipThis work was supported by the Mycobacteriology Unit of Instituteof Tropical Medicine, Antwerp, Belgium and interuniversity coopera-tion between Jimma University and Flemish Universities (VLIR-OUSproject).en_US
dc.language.isoenen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectMycobacterium bovisen_US
dc.subjectTuberculous lymphadenitisen_US
dc.subjectEthiopiaen_US
dc.titleThe predominance of Ethiopian specific Mycobacterium tuberculosis families and minimal contribution of Mycobacterium bovis in tuberculous lymphadenitis patients in Southwest Ethiopiaen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-09-13
dc.date.application2017-09-15
dc.typeArticleen_US
dc.type.versionNo full-text in the repositoryen_US
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2017.09.016
dc.date.updated2019-09-24T09:46:05Z


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