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dc.contributor.authorEddyani, M.
dc.contributor.authorVandelannoote, K.
dc.contributor.authorMeehan, Conor J.
dc.contributor.authorBhuju, S.
dc.contributor.authorPorter, J.L.
dc.contributor.authorAguiar, J.
dc.contributor.authorSeemann, T.
dc.contributor.authorJarek, M.
dc.contributor.authorSingh, M.
dc.contributor.authorPortaels, F.
dc.contributor.authorStinear, T.P.
dc.contributor.authorde Jong, B.C.
dc.date.accessioned2019-09-24T10:38:59Z
dc.date.accessioned2019-10-10T13:28:04Z
dc.date.available2019-09-24T10:38:59Z
dc.date.available2019-10-10T13:28:04Z
dc.date.issued2015-11-30
dc.identifier.citationEddyani M, Vandelannoote K, Meehan CJ et al (2015) A Genomic Approach to Resolving Relapse versus Reinfection among Four Cases of Buruli Ulcer. PLoS Neglected Tropical Diseases, 9 (11): e0004158.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17297
dc.descriptionYesen_US
dc.description.abstractBackground. Increased availability of Next Generation Sequencing (NGS) techniques allows, for the first time, to distinguish relapses from reinfections in patients with multiple Buruli ulcer (BU) episodes. Methodology. We compared the number and location of single nucleotide polymorphisms (SNPs) identified by genomic screening between four pairs of Mycobacterium ulcerans isolates collected at the time of first diagnosis and at recurrence, derived from a collection of almost 5000 well characterized clinical samples from one BU treatment center in Benin. Principal Findings. The findings suggest that after surgical treatment—without antibiotics—the second episodes were due to relapse rather than reinfection. Since specific antibiotics were introduced for the treatment of BU, the one patient with a culture available from both disease episodes had M. ulcerans isolates with a genomic distance of 20 SNPs, suggesting the patient was most likely reinfected rather than having a relapse. Conclusions. To our knowledge, this study is the first to study recurrences in M. ulcerans using NGS, and to identify exogenous reinfection as causing a recurrence of BU. The occurrence of reinfection highlights the contribution of ongoing exposure to M. ulcerans to disease recurrence, and has implications for vaccine development.en_US
dc.description.sponsorshipThis work was supported by the UBS Optimus Foundation (Zurich, Switzerland) and the Department of Economy, Science and Innovation of the Flemish Government (Belgium). KV was supported by a VLADOC PhD scholarship of VLIRUOS (Belgium).en_US
dc.language.isoenen_US
dc.rights© 2015 Eddyani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0, https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectBuruli ulceren_US
dc.subjectRelapseen_US
dc.subjectReinfectionen_US
dc.subjectNext Generation Sequencingen_US
dc.subjectGenomic screeningen_US
dc.titleA Genomic Approach to Resolving Relapse versus Reinfection among Four Cases of Buruli Ulceren_US
dc.status.refereedYesen_US
dc.date.Accepted2015-09-22
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0004158
dc.date.updated2019-09-24T09:39:00Z
refterms.dateFOA2019-10-10T13:31:30Z


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