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dc.contributor.authorBachmann, J.A.
dc.contributor.authorTedder, Andrew
dc.contributor.authorLaenen, B.
dc.contributor.authorFracassetti, M.
dc.contributor.authorDésamoré, A.
dc.contributor.authorLafon-Placette, C.
dc.contributor.authorSteige, K.A.
dc.contributor.authorCallot, C.
dc.contributor.authorMarande, W.
dc.contributor.authorNeuffer, B.
dc.contributor.authorBergès, H.
dc.contributor.authorKöhler, C.
dc.contributor.authorCastric, V.
dc.contributor.authorSlotte, T.
dc.date.accessioned2019-09-13T10:04:26Z
dc.date.accessioned2019-09-27T12:22:29Z
dc.date.available2019-09-13T10:04:26Z
dc.date.available2019-09-27T12:22:29Z
dc.date.issued2019-06
dc.identifier.citationBachmann JA, Tedder A, Laenen B et al (2019) Genetic basis and timing of a major mating system shift in Capsella. New Phytologist, 224: 505-517.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17270
dc.descriptionYesen_US
dc.description.abstractA crucial step in the transition from outcrossing to self-fertilization is the loss of genetic self-incompatibility (SI). In the Brassicaceae, SI involves the interaction of female and male speci-ficity components, encoded by the genesSRKandSCRat the self-incompatibility locus (S-lo-cus). Theory predicts thatS-linked mutations, and especially dominant mutations inSCR, arelikely to contribute to loss of SI. However, few studies have investigated the contribution ofdominant mutations to loss of SI in wild plant species. Here, we investigate the genetic basis of loss of SI in the self-fertilizing crucifer speciesCapsella orientalis, by combining genetic mapping, long-read sequencing of completeS-hap-lotypes, gene expression analyses and controlled crosses. We show that loss of SI inC. orientalisoccurred<2.6 Mya and maps as a dominant trait totheS-locus. We identify a fixed frameshift deletion in the male specificity geneSCRand con-firm loss of male SI specificity. We further identify anS-linked small RNA that is predicted tocause dominance of self-compatibility. Our results agree with predictions on the contribution of dominantS-linked mutations toloss of SI, and thus provide new insights into the molecular basis of mating system transitions.en_US
dc.description.sponsorshipWork at Uppsala Genome Center is funded by 550 RFI / VR and Science for Life Laboratory, Sweden. The SNP&SEQ Platform is supported by 551 the Swedish Research Council and the Knut and Alice Wallenberg Foundation. V.C. 552 acknowledges support by a grant from the European Research Council (NOVEL project, 553 grant #648321). The authors thank the French Ministère de l’Enseignement Supérieur et de la 554 Recherche, the Hauts de France Region and the European Funds for Regional Economical 555 Development for their financial support to this project. This work was supported by a grant 556 from the Swedish Research Council (grant #D0432001) and by a grant from the Science for 557 Life Laboratory, Swedish Biodiversity Program to T.S. The Swedish Biodiversity Program is 558 supported by the Knut and Alice Wallenberg Foundation.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1111/nph.16035en_US
dc.rights© 2019 The Authors New Phytologist. © 2019 New Phytologist Trust. This is the peer reviewed version of the following article: Bachmann JA, Tedder A, Laenen B et al (2019) Genetic basis and timing of a major mating system shift in Capsella. New Phytologist, 224: 505-517, which has been published in final form at https://doi.org/10.1111/nph.16035. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
dc.subjectCapsellaen_US
dc.subjectDominance modifieren_US
dc.subjectLong-read sequencingen_US
dc.subjectParallel evolutionen_US
dc.subjectPlant 45 mating system shiften_US
dc.subjectSelf-compatibilityen_US
dc.subjectS-locusen_US
dc.subjectSmall RNAen_US
dc.titleGenetic basis and timing of a major mating system shift in Capsellaen_US
dc.status.refereedYesen_US
dc.date.Accepted2019-06-20
dc.date.application2019-06-29
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
dc.date.updated2019-09-13T09:04:32Z
refterms.dateFOA2019-09-27T12:22:59Z


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