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dc.contributor.authorJones, Alfred N.
dc.contributor.authorBridgeman, John
dc.date.accessioned2019-09-03T11:37:17Z
dc.date.accessioned2019-09-24T11:28:11Z
dc.date.available2019-09-03T11:37:17Z
dc.date.available2019-09-24T11:28:11Z
dc.date.issued2019-11
dc.identifier.citationJones AN and Bridgeman J (2019) Identifying molecular mass of coagulant protein from edible Hibiscus seeds using SDS-PAGE analysis. Journal of the Environmental Engineering Division. 145(11)en_US
dc.identifier.urihttp://hdl.handle.net/10454/17249
dc.descriptionYesen_US
dc.description.abstractThis study used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and a jar test apparatus to investigate the molecular weight (MW) and turbidity removal potential of Hibiscus seeds. Three Hibiscus species were assessed: okra crude extract (OCE), sabdariffa crude extract (SCE), and kenaf crude extract (KCE). Furthermore, purified versions of each [i.e., purified okra protein (POP), purified sabdariffa protein (PSP), and purified kenaf protein (PKP)] obtained from anionic exchange were evaluated. The results show that whereas the crude extracts had multiple proteins with MW sizes between 11 and 82 kDa, the purified samples consisted of a single coagulant protein band around 39 kDa. In each case, significant turbidity removal was recorded with the purified proteins; POP, PSP and PKP achieved approximately 98%, 94%, and 90% removal, respectively, at a reduced dosage of ≤0.6  mg/L. However, OCE and SCE achieved lower turbidity removal of 86% and 85% using 40-mg/L doses, respectively, whereas KCE recorded only 73% turbidity removal with a 60-mg/L dose. Sludge generation by crude and purified proteins was approximately 25% of sludge produced by aluminum sulfate and had the additional benefit of being biodegradable. Therefore, the coagulant protein in Hibiscus plant seeds has potential applications for improvements to accessing clean water in developing countries.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1061/(ASCE)EE.1943-7870.0001595en_US
dc.rights(c) 2019 ASCE. This material may be downloaded for personal use only. Any other use requires prior permission of the American Society of Civil Engineers. This material may be found at https://doi.org/10.1061/(ASCE)EE.1943-7870.0001595.en_US
dc.subjectProteinen_US
dc.subjectHibiscus seedsen_US
dc.subjectTurbidity removalen_US
dc.subjectMolecular weighten_US
dc.subjectWater treatmenten_US
dc.titleIdentifying molecular mass of coagulant protein from edible Hibiscus seeds using SDS-PAGE analysisen_US
dc.status.refereedYesen_US
dc.date.Accepted2019-03
dc.date.application2019-08-27
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.date.updated2019-09-03T10:37:28Z
refterms.dateFOA2019-09-24T11:29:54Z


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