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dc.contributor.authorHunt, L.J.
dc.contributor.authorAhmed, E.A.
dc.contributor.authorKaur, H.
dc.contributor.authorAhuja, J.S.
dc.contributor.authorHulme, L.
dc.contributor.authorChou, T.C.
dc.contributor.authorLichten, M.
dc.contributor.authorGoldman, Alastair S.H.
dc.date.accessioned2019-05-14T08:10:15Z
dc.date.available2019-05-14T08:10:15Z
dc.date.issued2019
dc.identifier.citationHunt LJ, Ahmed EA, Kaur H et al (2019) S. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregates. Chromosoma. Accepted for publication.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17048
dc.descriptionYesen_US
dc.description.abstractWe investigated the meiotic role of Srs2, a multi-functional DNA helicase/translocase that destabilises Rad51-DNA filaments and is thought to regulate strand invasion and prevent hyper-recombination during the mitotic cell cycle. We find that Srs2 activity is required for normal meiotic progression and spore viability. A significant fraction of srs2 mutant cells progress through both meiotic divisions without separating the bulk of their chromatin, although in such cells sister centromeres often separate. Undivided nuclei contain aggregates of Rad51 colocalised with the ssDNA-binding protein RPA, suggesting the presence of persistent single-strand DNA. Rad51 aggregate formation requires Spo11-induced DSBs, Rad51 strand-invasion activity and progression past the pachytene stage of meiosis, but not the DSB end-resection or the bias towards interhomologue strand invasion characteristic of normal meiosis. srs2 mutants also display altered meiotic recombination intermediate metabolism, revealed by defects in the formation of stable joint molecules. We suggest that Srs2, by limiting Rad51 accumulation on DNA, prevents the formation of aberrant recombination intermediates that otherwise would persist and interfere with normal chromosome segregation and nuclear division.en_US
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BB/K009346/1)en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1007/s00412-019-00705-9en_US
dc.rights(c) 2019 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectBudding yeasten_US
dc.subjectMeiosisen_US
dc.subjectRad51en_US
dc.subjectRecombinationen_US
dc.subjectSrs2 helicaseen_US
dc.titleS. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregatesen_US
dc.status.refereedYesen_US
dc.date.Accepted2019-04-24
dc.date.application2019-05-09
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2019-05-14T08:10:15Z


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