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    S. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregates

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    Publication date
    2019-09
    Author
    Hunt, L.J.
    Ahmed, E.A.
    Kaur, H.
    Ahuja, J.S.
    Hulme, L.
    Chou, T.C.
    Lichten, M.
    Goldman, Alastair S.H.
    Keyword
    Budding yeast
    Meiosis
    Rad51
    Recombination
    Srs2 helicase
    Rights
    (c) 2019 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    We investigated the meiotic role of Srs2, a multi-functional DNA helicase/translocase that destabilises Rad51-DNA filaments and is thought to regulate strand invasion and prevent hyper-recombination during the mitotic cell cycle. We find that Srs2 activity is required for normal meiotic progression and spore viability. A significant fraction of srs2 mutant cells progress through both meiotic divisions without separating the bulk of their chromatin, although in such cells sister centromeres often separate. Undivided nuclei contain aggregates of Rad51 colocalised with the ssDNA-binding protein RPA, suggesting the presence of persistent single-strand DNA. Rad51 aggregate formation requires Spo11-induced DSBs, Rad51 strand-invasion activity and progression past the pachytene stage of meiosis, but not the DSB end-resection or the bias towards interhomologue strand invasion characteristic of normal meiosis. srs2 mutants also display altered meiotic recombination intermediate metabolism, revealed by defects in the formation of stable joint molecules. We suggest that Srs2, by limiting Rad51 accumulation on DNA, prevents the formation of aberrant recombination intermediates that otherwise would persist and interfere with normal chromosome segregation and nuclear division.
    URI
    http://hdl.handle.net/10454/17048
    Version
    Published version
    Citation
    Hunt LJ, Ahmed EA, Kaur H et al (2019) S. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregates. Chromosoma. 128(3): 249-265.
    Link to publisher’s version
    https://doi.org/10.1007/s00412-019-00705-9
    Type
    Article
    Collections
    Life Sciences Publications

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