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    The effect of WIN55, 212-2 on protein S100, matrix metalloproteinase-2 and nitric oxide expression of chondrocyte monolayer

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    Sefat_SM_Journal_of_Biomedical_Engineering.pdf (2.514Mb)
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    Publication date
    2017-01
    Author
    Abdeldayum, Ali I.A.
    Youseffi, Mansour
    Sefat, Farshid
    Genedy, Mohamed A.
    Abdul Jamil, M.M.
    Javid, F.
    Keyword
    Chondrocyte
    WIN55
    212-2
    Wound healing
    Protein expression
    Collagen type-I and II
    Fibronectin
    MMP-2 and NO expression
    Rights
    © 2017 M Youseffi. This work is licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Studies have been conducted to highlight the anti-inflammatory and immunosuppressive properties of synthetic cannabinoids as well as their potential for cartilage repair. Various wound healing techniques can be used to investigate the mechanisms of chondrocyte repair in monolayers or three dimensional tissues constructs. In this work the effect of WIN55, 212-2 (WIN-2) on nitric oxide (NO) and matrix metalloproteinase-2 (MMP-2) expressed by wounded chondrocyte monolayers was investigated. Moreover, expression of collagen type-I and type-II, fibronectin and S100 proteins were detected using immunofluorescence and quantitatively verified using ELISA based techniques following treatment with 1 μM and 2 μM of WIN-2. Treating chondrocytes with 1 μM of WIN-2 significantly increased expression of collagen type-II, fibronectin and S100, and significantly reduced collagen type-I expressions as compared to the control groups. On the other hand, both concentrations of WIN-2 significantly reduced the expression of the inflammation markers NO and MMP-2 in a dose dependent manner. These findings highlight the potential use of the synthetic cannabinoids for improving cartilage healing properties as well as acting as an anti-inflammatory agent which could be used to enhance tissue engineering protocols aimed at cartilage repair.
    URI
    http://hdl.handle.net/10454/17016
    Version
    Published version
    Citation
    Abdeldayum A, Youseffi M, Sefat F et al (2017) The effect of WIN55, 212-2 on protein S100, matrix metalloproteinase-2 and nitric oxide expression of chondrocyte monolayer. SM Journal of Biomedical Engineering. 3(1): 1011.
    Link to publisher’s version
    https://smjournals.com/biomedical-engineering/repository.php
    Type
    Article
    Collections
    Engineering and Informatics Publications

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