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    Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity

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    Publication date
    2016-03-15
    Author
    Hamed, Refaat B.
    Gomez-Castellanos, J.R.
    Sean Froese, D.
    Krysztofinska, E.
    Yue, W.W.
    Schofield, C.J.
    Keyword
    Antibiotics
    Biosynthesis
    Carbapenem
    Crotonases
    Dynamic kinetic discrimination
    Enolate reactivity
    Stereoselectivity
    Peer-Reviewed
    Yes
    
    Metadata
    Show full item record
    Abstract
    The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.
    URI
    http://hdl.handle.net/10454/17012
    Version
    No full-text in the repository
    Citation
    Hamed RB, Gomez-castellanos JR, Sean Froese D et al (2016) Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity. ChemBioChem. 17(6): 471-473.
    Link to publisher’s version
    https://doi.org/10.1002/cbic.201500644
    Type
    Article
    Collections
    Life Sciences Publications

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