Show simple item record

dc.contributor.authorSharma, S.V.*
dc.contributor.authorTong, X.*
dc.contributor.authorPubill-Ulldemolins, C.*
dc.contributor.authorCartmell, C.*
dc.contributor.authorBogosyan, E.J.A.*
dc.contributor.authorRackham, E.J.*
dc.contributor.authorMarelli, E.*
dc.contributor.authorHamed, Refaat B.*
dc.contributor.authorGoss, R.J.M.*
dc.date.accessioned2019-05-03T12:41:14Z
dc.date.available2019-05-03T12:41:14Z
dc.date.issued2017-08-09
dc.identifier.citationSharma SV, Tong X, Pubill-Ulldemolins C et al (2017) Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo. Nature Communications. 8: 229.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17009
dc.descriptionYesen_US
dc.description.abstractMarrying synthetic biology with synthetic chemistry provides a powerful approach toward natural product diversification, combining the best of both worlds: expediency and synthetic capability of biogenic pathways and chemical diversity enabled by organic synthesis. Biosynthetic pathway engineering can be employed to insert a chemically orthogonal tag into a complex natural scaffold affording the possibility of site-selective modification without employing protecting group strategies. Here we show that, by installing a sufficiently reactive handle (e.g., a C–Br bond) and developing compatible mild aqueous chemistries, synchronous biosynthesis of the tagged metabolite and its subsequent chemical modification in living culture can be achieved. This approach can potentially enable many new applications: for example, assay of directed evolution of enzymes catalyzing halo-metabolite biosynthesis in living cells or generating and following the fate of tagged metabolites and biomolecules in living systems. We report synthetic biological access to new-to-nature bromo-metabolites and the concomitant biorthogonal cross-coupling of halo-metabolites in living cultures.en_US
dc.description.sponsorshipEuropean Research Council under the European Union’s Seventh Framework Programme (FP7/2007–2013/ERC consolidator grant GCGXC grant agreement no 614779) and ERAIB (Grant no. 031A338A) and H2020-MSCA-IF-2014 Grant no. 659399en_US
dc.language.isoenen_US
dc.rights(c) 2017 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectGenoChemeticsen_US
dc.subjectSynthetic biologyen_US
dc.subjectSynthetic chemistryen_US
dc.subjectBiogenic pathwaysen_US
dc.subjectOrganic synthesisen_US
dc.titleLiving GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivoen_US
dc.status.refereedYesen_US
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.identifier.doihttps://doi.org/10.1038/s41467-017-00194-3
refterms.dateFOA2019-05-03T12:41:14Z


Item file(s)

Thumbnail
Name:
sharma_et_al_2017.pdf
Size:
1.145Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record