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dc.contributor.authorGentile, P.*
dc.contributor.authorMcColgan-Bannon, K.*
dc.contributor.authorGianone, N.C.*
dc.contributor.authorSefat, Farshid*
dc.contributor.authorDalgarno, K.*
dc.contributor.authorFerreira, A.M.*
dc.date.accessioned2019-04-30T13:05:09Z
dc.date.available2019-04-30T13:05:09Z
dc.date.issued2017-06
dc.identifier.citationGentile P, McColgan-Bannon K, Gianone NC et al (2017) Biosynthetic PCL-graft-collagen bulk material for tissue engineering applications. Materials. 10(7): 693.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16998
dc.descriptionYesen_US
dc.description.abstractBiosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning.en_US
dc.description.sponsorshipThe EPSRC Centre in Innovative Manufacturing in Medical Devices (MeDe Innovation; EP/K029592/1).en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.3390/ma10070693en_US
dc.rights© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectBiosyntheticen_US
dc.subjectCollagenen_US
dc.subjectPoly("-caprolactone)en_US
dc.subjectConjugationen_US
dc.subjectElectrospinningen_US
dc.subjectTissue engineeringen_US
dc.titleBiosynthetic PCL-graft-collagen bulk material for tissue engineering applicationsen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-06-19
dc.date.application2017-06-23
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2019-04-30T13:05:09Z


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