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dc.contributor.authorJamil, M. Mahadi Abdul*
dc.contributor.authorZaltum, M.A.M.*
dc.contributor.authorRahman, N.A.A.*
dc.contributor.authorAmbar, R.*
dc.contributor.authorDenyer, Morgan C.T.*
dc.contributor.authorJaved, F.*
dc.contributor.authorSefat, Farshid*
dc.contributor.authorMozafari, M.*
dc.contributor.authorYouseffi, Mansour*
dc.date.accessioned2019-04-30T10:58:17Z
dc.date.available2019-04-30T10:58:17Z
dc.date.issued2018
dc.identifier.citationJamil MMA, Zaltum MAM, Rahman NAA et al (2018) Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface. Journal of Physics: Conference Series. 1019(1) 012018.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16996
dc.descriptionYesen_US
dc.description.abstractCell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of 25μm, 50μm and 100μm width. However, HeLa cells seeded on 50μm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50μm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100μs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachment and cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.en_US
dc.description.sponsorshipMinistry of Higher Education Malaysia and UTHM Tier 1 Research Grant (U865)en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1088/1742-6596/1019/1/012018en_US
dc.rightsPublished under licence in Journal of Physics: Conference Series by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (or successor licence). Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.en_US
dc.subjectHeLaen_US
dc.subjectCells alignmenten_US
dc.subjectPulse electric fielden_US
dc.subjectMicro contact printingen_US
dc.subjectExtra cellular matrixen_US
dc.subjectCell proliferationen_US
dc.subjectCell guidanceen_US
dc.titleInvestigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surfaceen_US
dc.status.refereedYesen_US
dc.date.application2018-06-27
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2019-04-30T10:58:17Z


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