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dc.contributor.authorRaja, Tehmeena I.*
dc.contributor.authorKhaghani, Seyed A.*
dc.contributor.authorZafar, M.S.*
dc.contributor.authorKhurshid, Z.*
dc.contributor.authorMozafari, M.*
dc.contributor.authorYouseffi, Mansour*
dc.contributor.authorSefat, Farshid*
dc.date.accessioned2019-04-29T08:52:15Z
dc.date.available2019-04-29T08:52:15Z
dc.date.issued2018
dc.identifier.citationRaja TI, Khaghani SA, Zafar MS et al (2018) Effect of TGF-β1 on water retention properties of healthy and osteoarthritic chondrocytes. Materials Today: Proceedings. 7(3) Part 3: 15717-15725.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16988
dc.descriptionYesen_US
dc.description.abstractArticular cartilage, a connective tissue, contains chondrocytes and glycosaminoglycans (GAGs) which aid in water retention, providing the tissue with its magnificent ability to prevent friction, withstand loads and absorb compressive shocks however, cartilage, does not have the ability to regenerate and repair. Osteoarthritis (OA) is a progressive degenerative disease, which includes reduction of cartilage thickness between two bones in a joint, causing painful bone-to-bone contact. OA affects over 8 million people in the UK alone. , and as the primary causes are unknown, available treatments including surgical and non-surgical techniques which only reduce the symptoms created by the disorder instead of providing a cure. This project focused on utilizing TGF-β1, a cytokine found in elevated amounts in healthy cartilage when compared to degraded cartilage, in order to observe the effects of the growth factor on both healthy and osteoarthritic chondrocytes. The healthy and the osteoarthritic chondrocytes were cultured in two different media (DMEM with and without TGF- β1) before utilizing the SpectraMax M2/M2e plate reader to observe and analyze the effect of TGF-β1 on water retention properties of cells. This has been achieved by quantifying the GAG content using DMMB dye. Results showed that although TGF-β1 did displayed an increase in glycosaminoglycan synthesis, the statistical increase was not vast enough for the alternative hypothesis to be accepted; further experimentation with TGF-β1, alongside other cytokines within the growth factor family is needed to perceive the true influence of the growth factor on un cured degenerative diseases. It was concluded that both the healthy and osteoarthritic cells treated with TGF-β1 absorbed considerably more DMMB in comparison to the cells, suggesting that TGF-β1 indeed works to aid in water retention. TGF-β1 is a key factor to be exploited when constructing treatments for osteoarthritis
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.matpr.2018.04.183en_US
dc.rights© 2018 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.subjectTGF- β1en_US
dc.subjectCytokinesen_US
dc.subjectWater retentionen_US
dc.subjectArticular cartilegeen_US
dc.subjectChondrocytesen_US
dc.subjectOsteoarthritisen_US
dc.subjectImmunocytochemistryen_US
dc.subjectDMMBen_US
dc.titleEffect of TGF-β1 on water retention properties of healthy and osteoarthritic chondrocytesen_US
dc.status.refereedYesen_US
dc.date.Accepted2018
dc.date.application2018-06-08
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US


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