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    Effect of TGF-β1 on water retention properties of healthy and osteoarthritic chondrocytes

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    Publication date
    2018
    Author
    Raja, Tehmeena I.
    Khaghani, Seyed A.
    Zafar, M.S.
    Khurshid, Z.
    Mozafari, M.
    Youseffi, Mansour
    Sefat, Farshid
    Keyword
    TGF- β1
    Cytokines
    Water retention
    Articular cartilege
    Chondrocytes
    Osteoarthritis
    Immunocytochemistry
    DMMB
    Rights
    © 2018 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
    Peer-Reviewed
    Yes
    
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    Abstract
    Articular cartilage, a connective tissue, contains chondrocytes and glycosaminoglycans (GAGs) which aid in water retention, providing the tissue with its magnificent ability to prevent friction, withstand loads and absorb compressive shocks however, cartilage, does not have the ability to regenerate and repair. Osteoarthritis (OA) is a progressive degenerative disease, which includes reduction of cartilage thickness between two bones in a joint, causing painful bone-to-bone contact. OA affects over 8 million people in the UK alone. , and as the primary causes are unknown, available treatments including surgical and non-surgical techniques which only reduce the symptoms created by the disorder instead of providing a cure. This project focused on utilizing TGF-β1, a cytokine found in elevated amounts in healthy cartilage when compared to degraded cartilage, in order to observe the effects of the growth factor on both healthy and osteoarthritic chondrocytes. The healthy and the osteoarthritic chondrocytes were cultured in two different media (DMEM with and without TGF- β1) before utilizing the SpectraMax M2/M2e plate reader to observe and analyze the effect of TGF-β1 on water retention properties of cells. This has been achieved by quantifying the GAG content using DMMB dye. Results showed that although TGF-β1 did displayed an increase in glycosaminoglycan synthesis, the statistical increase was not vast enough for the alternative hypothesis to be accepted; further experimentation with TGF-β1, alongside other cytokines within the growth factor family is needed to perceive the true influence of the growth factor on un cured degenerative diseases. It was concluded that both the healthy and osteoarthritic cells treated with TGF-β1 absorbed considerably more DMMB in comparison to the cells, suggesting that TGF-β1 indeed works to aid in water retention. TGF-β1 is a key factor to be exploited when constructing treatments for osteoarthritis
    URI
    http://hdl.handle.net/10454/16988
    Version
    Accepted manuscript
    Citation
    Raja TI, Khaghani SA, Zafar MS et al (2018) Effect of TGF-β1 on water retention properties of healthy and osteoarthritic chondrocytes. Materials Today: Proceedings. 7(3) Part 3: 15717-15725.
    Link to publisher’s version
    https://doi.org/10.1016/j.matpr.2018.04.183
    Type
    Article
    Collections
    Engineering and Informatics Publications

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