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dc.contributor.authorBridgeman, John*
dc.contributor.authorBaker, A.*
dc.contributor.authorBrown, D.*
dc.contributor.authorBoxall, J.B.*
dc.date.accessioned2019-04-15T12:40:38Z
dc.date.available2019-04-15T12:40:38Z
dc.date.issued2015
dc.identifier.citationBridgeman J, Baker A, Brown D et al (2015) Portable LED fluorescence instrumentation for the rapid assessment of potable water quality. Science of the Total Environment. 524-525: 338-346.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16965
dc.descriptionYesen_US
dc.description.abstractCharacterising the organic and microbial matrix of water are key issues in ensuring a safe potable water supply. Current techniques only confirm water quality retrospectively via laboratory analysis of discrete samples. Whilst such analysis is required for regulatory purposes, it would be highly beneficial to monitor water quality in-situ in real time, enabling rapid water quality assessment and facilitating proactive management of water supply systems. A novel LED-based instrument, detecting fluorescence peaks C and T (surrogates for organic and microbial matter, respectively), was constructed and performance assessed. Results from over 200 samples taken from source waters through to customer tap from three UK water companies are presented. Excellent correlation was observed between the new device and a research grade spectrophotometer (r2 = 0.98 and 0.77 for peak C and peak T respectively), demonstrating the potential of providing a low cost, portable alternative fluorimeter. The peak C/TOC correlation was very good (r 2 = 0.75) at low TOC levels found in drinking water. However, correlations between peak T and regulatory measures of microbial matter (2 day/3 day heterotrophic plate counts (HPC), E. coli, and total coliforms) were poor, due to the specific nature of these regulatory measures and the general measure of peak T. A more promising correlation was obtained between peak T and total bacteria using flow cytometry. Assessment of the fluorescence of four individual bacteria isolated from drinking water was also considered and excellent correlations found with peak T (Sphingobium sp. (r 2 = 0.83); Methylobacterium sp. (r 2 = 1.0); Rhodococcus sp. (r 2 = 0.86); Xenophilus sp. (r 2 = 0.96)). It is notable that each of the bacteria studied exhibited different levels of fluorescence as a function of their number. The scope for LED based instrumentation for insitu, real time assessment of the organic and microbial matrix of potable water is clearly demonstrated.en_US
dc.description.sponsorshipThe research reported here was sponsored by the Engineering and Physical Sciences Research Council under grants EP/I001379/1, EP/I001468/1 and EP/I029346/1.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.scitotenv.2015.04.050en_US
dc.rights© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectFluorescenceen_US
dc.subjectLEDsen_US
dc.subjectOrganic matteren_US
dc.subjectMicrobial matteren_US
dc.subjectPotable water qualityen_US
dc.titlePortable LED fluorescence instrumentation for the rapid assessment of potable water qualityen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-04-13
dc.date.application2015-04-22
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2019-04-15T12:40:38Z


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