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    Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques

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    Publication date
    2018-02
    Author
    Soon, C.F.
    Tee, K.S.
    Wong, S.C.
    Nayan, N.
    Sundra, S.
    Ahmad, M.K.
    Sefat, Farshid
    Sultana, N.
    Youseffi, Mansour
    Keyword
    3D cell culture
    Biophysical properties
    Liquid crystal
    Microtissues
    Keratinocytes
    Microencapsulation
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 μm and 141 ± 70 μm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications.
    URI
    http://hdl.handle.net/10454/16942
    Version
    No full-text in the repository
    Citation
    Soon CF, Tee KS, Wong SC et al (2018) Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques. Cytotechnology. 70(1): 13-29.
    Link to publisher’s version
    https://doi.org/10.1007/s10616-017-0168-2
    Type
    Article
    Collections
    Engineering and Informatics Publications

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