Show simple item record

dc.contributor.authorLeong, W.Y.*
dc.contributor.authorSoon, C.F.*
dc.contributor.authorWong, S.C.*
dc.contributor.authorTee, K.S.*
dc.contributor.authorCheong, S.C.*
dc.contributor.authorGan, S.H.*
dc.contributor.authorYouseffi, Mansour*
dc.date.accessioned2019-04-01T13:35:12Z
dc.date.available2019-04-01T13:35:12Z
dc.date.issued2017-05
dc.identifier.citationLeong WY, Soon CF, Wong SC et al (2017) In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique. Bioengineering. 4(2): 43.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16939
dc.descriptionYesen_US
dc.description.abstractCells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.en_US
dc.description.sponsorshipMalaysia Ministry of Education (Fundamental Research Grant Scheme, FRGS Vot. 1482 and IGSP Vot. 679).en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.3390/bioengineering4020043en_US
dc.rights© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectAlginateen_US
dc.subjectAerosolen_US
dc.subjectMicroencapsulationen_US
dc.subjectMicrotissuesen_US
dc.subjectKeratinocytesen_US
dc.subjectOral squamous cell carcinomaen_US
dc.titleIn vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation techniqueen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-05-12
dc.date.application2017-05-14
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2019-04-01T13:35:12Z


Item file(s)

Thumbnail
Name:
Youseffi_Bioengineering.pdf
Size:
9.805Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record