Show simple item record

dc.contributor.authorKhaghani, Seyed A.*
dc.contributor.authorAkbarova, G.*
dc.contributor.authorSoon, C.F.*
dc.contributor.authorDilbazi, G.*
dc.date.accessioned2018-11-13T15:03:09Z
dc.date.available2018-11-13T15:03:09Z
dc.date.issued2018-12
dc.identifier.citationKhaghani SA, Akbarova G, Soon CF et al (2018) Effect of transforming growth factor-β2 on biological regulation of multilayer primary chondrocyte culture. Cell and Tissue Banking. 19(4): 763-775.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16648
dc.descriptionYesen_US
dc.description.abstractCytokines are extremely potent biomolecules that regulate cellular functions and play multiple roles in initiation and inhibition of disease. These highly specialised macromolecules are actively involved in control of cellular proliferation, apoptosis, cell migration and adhesion. This work, investigates the effect of transforming growth factor-beta2 (TGF-β2) on the biological regulation of chondrocyte and the repair of a created model wound on a multilayer culture system. Also the effect of this cytokine on cell length, proliferation, and cell adhesion has been investigated. Chondrocytes isolated from knee joint of rats and cultured at 4 layers. Each layer consisted of 2 × 105 cells/ml with and without TGF-β2. The expression of mRNA and protein levels of TGF-β receptors and Smad1, 3, 4, and 7 have been analysed by RT-PCR and western blot analysis. The effect of different supplementations in chondrocyte cell proliferation, cell length, adhesion, and wound repair was statistically analysed by One-way ANOVA test. Our results showed that the TGFβ2 regulates mRNA levels of its own receptors, and of Smad3 and Smad7. Also the TGF-β2 caused an increase in chondrocyte cell length, but decreased its proliferation rate and the wound healing process. TGF-β2 also decreased cell adhesion ability to the surface of the culture flask. Since, TGF-β2 increased the cell size, but showed negative effect on cell proliferation and adhesion of CHC, the effect of manipulated TGF-β2 with other growth factors and/or proteins needs to be investigated to finalize the utilization of this growth factor and design of scaffolding in treatment of different types of arthritis.en_US
dc.language.isoenen_US
dc.rights(c) 2018 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectChondrocyteen_US
dc.subjectCytokinesen_US
dc.subjectTGF-β2en_US
dc.subjectGene expressionsen_US
dc.subjectWound repairen_US
dc.subjectMultilayer cell cultureen_US
dc.titleEffect of transforming growth factor-β2 on biological regulation of multilayer primary chondrocyte cultureen_US
dc.status.refereedYesen_US
dc.date.Accepted2018-10-15
dc.date.application2018-10-30
dc.typeArticleen_US
dc.type.versionpublished version paperen_US
dc.identifier.doihttps://doi.org/10.1007/s10561-018-9732-z
refterms.dateFOA2018-11-13T15:03:09Z


Item file(s)

Thumbnail
Name:
khaghani_et_al_2018.pdf
Size:
1.359Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record