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dc.contributor.authorHabas, Khaled S.A.
dc.contributor.authorShang, Lijun
dc.date.accessioned2018-09-28T11:54:15Z
dc.date.available2018-09-28T11:54:15Z
dc.date.issued2018-10
dc.identifier.citationHabas K and Shang L (2018) Alterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cells. Tissue and Cell. 54: 139-143.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16587
dc.descriptionYesen_US
dc.description.abstractAlterations of Endothelial cells (ECs) play a critical role in different pathogenesis of many serious human diseases, and dysfunction of the vascular endothelium is an indicator for human disorders. Endothelial dysfunction is considered to be an early indicator for atherosclerosis, which is characterised by overexpression of adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Hydrogen peroxide (H2O2) released via neutrophils is an important mediator of endothelial cell function. Ambient production of superoxide anion (O2−) and subsequently H2O2 at low levels is critical for regulating endothelial cell functions and proliferation. In this study, we investigated the effects of H2O2 on the expression of adhesion molecules VCAM-1 and ICAM-1 in cultured human umbilical vein endothelial cells (HUVECs). Intracellular superoxide anion production was detected by using p-Nitro Blue Tetrazolium (NBT) assay. Our results showed that administration of 100μM of H2O2 on HUVECs for 2, 6, 12 and 24 h induced a time-dependent increase in ICAM-1 and VCAM-1 mRNA and protein expression levels with a significant increase observed from 6 h. HUVECs exposed to H2O2 exhibit increased O2−, suggesting that H2O2 induced oxidative stress may be a reasonable for atherosclerosis. This increase can be reduced by the flavonoid, N-acetyl cysteine (NAC). The modulation of endothelial cell function through this mechanism may underlie the contribution of H2O2 to the development of vascular disease.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.tice.2018.09.002en_US
dc.rightsCrown Copyright © 2018 Published by Elsevier Ltd. All rights reserved. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en_US
dc.subjectHuman umbilical vein endothelial cells (HUVECs)en_US
dc.subjectIntercellular adhesion molecule 1(CAM-1)en_US
dc.subjectVascular cell adhesion molecule 1 (VCAM-1)en_US
dc.subjectHydrogen peroxide (H2O2)en_US
dc.titleAlterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cellsen_US
dc.status.refereedYesen_US
dc.date.Accepted2018-09-11
dc.date.application2018-09-12
dc.typeArticleen_US
dc.date.EndofEmbargo2019-09-13
dc.type.versionAccepted Manuscripten_US
dc.description.publicnotesThe full-text of this article will be released for public view at the end of the publisher embargo on 13 Sep 2019.en_US
refterms.dateFOA2018-09-28T11:54:19Z


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