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dc.contributor.authorJoly-Tonetti, Nicolas*
dc.contributor.authorWibawa, J.I.D.*
dc.contributor.authorBell, M.*
dc.contributor.authorTobin, Desmond J.*
dc.date.accessioned2018-08-01T13:45:38Z
dc.date.available2018-08-01T13:45:38Z
dc.date.issued2018-11
dc.identifier.citationJoly-Tonetti N, Wibawa JID, Bell M et al (2018) An explanation for the mysterious distribution of melanin in human skin ‐ a rare example of asymmetric (melanin) organelle distribution during mitosis of basal layer progenitor keratinocytes. British Journal of Dermatology. 179(5): 1115-1126.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16519
dc.descriptionYesen_US
dc.description.abstractBackground: Melanin is synthesized by melanocytes in the basal layer of the epidermis. When transferred to surrounding keratinocytes it is the key UVR-protective biopolymer responsible for skin pigmentation. Most melanin is observable in the proliferative basal layer of the epidermis, and only sparsely distributed in the stratifying/differentiating epidermis. The latter has been explained, despite formal evidence, to ‘melanin degradation’ in supra-basal layers. Objectives: Our aim was to re-evaluate this currently-accepted basis for melanin distribution in the human skin epidermis, and whether this pattern is altered after a regenerative stimulus. Methods: Normal epidermis of adult human skin, at rest and after tape-stripping, was analysed by a range of (immuno)histochemical and high-resolution microscopy techniques. In vitro models of melanin granule uptake by human keratinocytes were attempted. Results: We propose a wholly different fate for melanin in the human epidermis. Our evidence indicates that the bulk of melanin is inherited only by the non-differentiating daughter cell post mitosis in progenitor keratinocytes, via asymmetric organelle inheritance. Moreover, this preferred pattern of melanin distribution can switch to a symmetric or equal daughter cell inheritance mode under conditions of stress including regeneration. Conclusions: We provide in this preliminary report a plausible and histologically-supportable explanation for how human skin pigmentation is efficiently organized in the epidermis. Steady state epidermis pigmentation may involve much less redox-sensitive melanogenesis than previously thought, and at least some pre-made melanin may be available for re-use. The epidermal-melanin unit may be an excellent example to study organelle distribution via asymmetric or symmetric inheritance in response to micro-environment and tissue demands.en_US
dc.description.sponsorshipWalgreens Boots Allianceen_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1111/bjd.16926en_US
dc.rights© 2018 Wiley This is the peer reviewed version of the following article: Joly-Tonetti N, Wibawa JID, Bell M et al (2018) An explanation for the mysterious distribution of melanin in human skin ‐ a rare example of asymmetric (melanin) organelle distribution during mitosis of basal layer progenitor keratinocytes. British Journal of Dermatology. 179(5): 1115-1126, which has been published in final form at https://doi.org/10.1111/bjd.16926. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en_US
dc.subjectMelaninen_US
dc.subjectAsymmetric organelle distributionen_US
dc.subjectHuman skin epidermisen_US
dc.subjectProgenitor keratinocytesen_US
dc.titleAn explanation for the mysterious distribution of melanin in human skin ‐ a rare example of asymmetric (melanin) organelle distribution during mitosis of basal layer progenitor keratinocytesen_US
dc.status.refereedYesen_US
dc.date.Accepted2018-05-28
dc.date.application2018-06-29
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-08-01T13:45:42Z


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