Show simple item record

dc.contributor.authorHabas, Khaled S.A.
dc.contributor.authorAbdulmwli, Mhamoued
dc.contributor.authorDemir, E.
dc.contributor.authorJacob, B.K.
dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorAnderson, Diana
dc.date.accessioned2018-07-09T13:24:13Z
dc.date.available2018-07-09T13:24:13Z
dc.date.issued2018-10
dc.identifier.citationHabas K, Abdulmwli M, Demir E et al (2018) DNA damage protection by bulk and nano forms of quercetin in lymphocytes of patients with chronic obstructive pulmonary disease exposed to the food mutagen 2-amino-3-methylimidazo [4,5-f]quinolone (IQ). Environmental Research. 166: 10-15.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16420
dc.descriptionYesen_US
dc.description.abstractChronic obstructive pulmonary disease (COPD) in humans, describes a group of lung conditions characterised by airflow limitation that is poorly reversible. The airflow limitation usually progresses slowly and is related to an abnormal inflammatory response of the lung to toxic particles. COPD is characterised by oxidative stress and an increased risk of lung carcinoma. The 2-amino-3-methylimidazo [4,5-f]quinoline (IQ) is one of a number of mutagenic/carcinogenic heterocyclic amines found mainly in well-cooked meats which are thus part of the regular diet. Antioxidants are very important in order to protect the cells against oxidative damage. The aim of the present study was to assess the effects of IQ on the level of DNA damage and susceptibility to a potent mutagen in peripheral blood cells of COPD patients. DNA damage and the frequency of micronuclei (MNi) were evaluated using the Comet and micronucleus assays, respectively. Differential expressions of both mRNA and protein of the endogenous antioxidant enzyme catalase were evaluated with quantitative polymerase chain reaction (qPCR) and Western blot analysis, respectively. Furthermore, the effect of bulk and nano forms of quercetin and their combination with IQ were examined. Results of the present study clearly demonstrated that MNi frequency in the peripheral blood lymphocytes exhibited a positive correlation with the DNA damage as evident from the different Comet assay parameters. Increase of the endogenous antioxidant catalase also showed there was a stimulation of this enzyme system by IQ. Whereas, the endogenous antioxidant quercetin significantly reduced oxidative stress in COPD patients and healthy individuals.en_US
dc.description.sponsorshipLibyan Governmenten_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.envres.2018.05.012en_US
dc.rights© 2018 Elsevier Inc. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en_US
dc.subject2-amino-3-methylimidazo [4; 5-f]quinolone; DNA damage; Endogenous antioxidant catalase; Exogenous antioxidant flavonoid quercetin; Micronucleien_US
dc.titleDNA damage protection by bulk and nano forms of quercetin in lymphocytes of patients with chronic obstructive pulmonary disease exposed to the food mutagen 2-amino-3-methylimidazo [4,5-f]quinolone (IQ)en_US
dc.status.refereedYesen_US
dc.date.Accepted2018-05-10
dc.date.application2018-05-25
dc.typeArticleen_US
dc.date.EndofEmbargo2019-05-26
dc.type.versionAccepted Manuscripten_US
dc.description.publicnotesThe full-text of this article will be released for public view at the end of the publisher embargo on 26 May 2019.en_US


Item file(s)

Thumbnail
Name:
Main Manuscript COPDIQ 30.05.2 ...
Size:
245.0Kb
Format:
Unknown
Description:
To keep suppressed
Thumbnail
Name:
Habas_Environmental_Research.pdf
Embargo:
2019-05-26
Size:
728.2Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record