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dc.contributor.authorDoroshenko, N.*
dc.contributor.authorRimmer, Stephen*
dc.contributor.authorHoskins, Richard*
dc.contributor.authorGarg, P.*
dc.contributor.authorSwift, Thomas*
dc.contributor.authorSpencer, H.L.M.*
dc.contributor.authorLord, Rianne M.*
dc.contributor.authorKatsikogianni, Maria G.*
dc.contributor.authorPownall, D.*
dc.contributor.authorMacNeil, S.*
dc.contributor.authorDouglas, C.W.I.*
dc.contributor.authorShepherd, J.*
dc.date.accessioned2018-07-04T11:11:17Z
dc.date.available2018-07-04T11:11:17Z
dc.date.issued2018
dc.identifier.citationDoroshenko N, Rimmer S, Hoskins R et al (2018) Antibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the cornea. Biomaterials Science. 6(8): 2101-2109.en_US
dc.identifier.urihttp://hdl.handle.net/10454/16402
dc.descriptionYesen_US
dc.description.abstractMicrobial keratitis can arise from penetrating injuries to the cornea. Corneal trauma promotes bacterial attachment and biofilm growth, which decrease the effectiveness of antimicrobials against microbial keratitis. Improved therapeutic efficacy can be achieved by reducing microbial burden prior to antimicrobial therapy. This paper assesses a highly-branched poly(N-isopropyl acrylamide) with vancomycin end groups (HB-PNIPAM-van), for reducing bacterial attachment and biofilm formation. The polymer lacked antimicrobial activity against Staphylococcus aureus, but significantly inhibited biofilm formation (p = 0.0008) on plastic. Furthermore, pre-incubation of S. aureus cells with HB-PNIPAM-van reduced cell attachment by 50% and application of HB-PNIPAM-van to infected ex vivo rabbit corneas caused a 1-log reduction in bacterial recovery, compared to controls (p = 0.002). In conclusion, HB-PNIPAM-van may be a useful adjunct to antimicrobial therapy in the treatment of corneal infections.en_US
dc.description.sponsorshipMedical Research Council and the Department of Biotechnology, India under grant number, MR/N50188/2.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1039/C8BM00201Ken_US
dc.rights© The Royal Society of Chemistry 2018. Open Access article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.en_US
dc.subjectMicrobial keratitisen_US
dc.subjectCorneaen_US
dc.subjectCorneal traumaen_US
dc.subjectAntimicrobial therapyen_US
dc.titleAntibiotic functionalised polymers reduce bacterial biofilm and bioburden in a simulated infection of the corneaen_US
dc.status.refereedYesen_US
dc.date.Accepted2018-05-17
dc.date.application2018-06-04
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-29T02:38:17Z


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