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dc.contributor.authorNguyen, M.A.*
dc.contributor.authorHughes, Zak E.*
dc.contributor.authorLiu, Y.*
dc.contributor.authorLi, Y.*
dc.contributor.authorSwihart, M.T.*
dc.contributor.authorKnecht, M.R.*
dc.contributor.authorWalsh, T.R.*
dc.date.accessioned2018-05-18T11:17:41Z
dc.date.available2018-05-18T11:17:41Z
dc.date.issued2018-05
dc.identifier.citationNguyen MA, Hughes ZE, Liu Y et al (2018) Peptide-mediated growth and dispersion of Au nanoparticles in water via sequence engineering. The Journal of Physical Chemistry C. 122(21): 11532-11542.
dc.identifier.urihttp://hdl.handle.net/10454/15946
dc.descriptionYes
dc.description.abstractThe use of peptides to nucleate, grow, and stabilize nanoparticles in aqueous media via non-covalent interactions offers new possibilities for creating functional, water-dispersed inorganic/organic hybrid materials, particularly for Au nanoparticles. Numerous previous studies have identified peptide sequences that both possess a strong binding affinity for Au surfaces and are capable of supporting nanoparticle growth in water. However, recent studies have shown that not all such peptide sequences can produce stable dispersions of these nanoparticles. Here, via integrated experiments and molecular modeling, we provide new insights into the many factors that influence Au nanoparticle growth and stabilization in aqueous media. We define colloidal stability by the absence of visible precipitation after at least 24 hours post-synthesis. We use binding affinity measurements, nanoparticle synthesis, characterization and stabilization assays, and molecular modeling, to investigate a set of sequences based on two known peptides with strong affinity for Au. This set of biomolecules is designed to probe specific sequence and context effects using both point mutations and global reorganization of the peptides. Our data confirm, for a broader range of sequences, that Au nanoparticle/peptide binding affinity alone is not predictive of peptide-mediated colloidal stability. By comparing nanoparticle stabilization assay outcomes with molecular simulations, we establish a correlation between the colloidal stability of the Au nanoparticles and the degree of conformational diversity in the surface-adsorbed peptides. Our findings suggest future routes to engineer peptide sequences for bio-based growth and dispersion of functional nanoparticles in aqueous media.
dc.description.sponsorshipAir Office of Scientific Research, grant number FA9550-12-1-0226.
dc.language.isoen
dc.rights© 2018 ACS. This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry C, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcc.8b02392
dc.subjectBio-nanotechnology
dc.subjectGold nanoparticles
dc.subjectPeptides
dc.subjectMolecular simulation
dc.subjectMutations
dc.subjectBinding affinity
dc.subjectStabilization
dc.titlePeptide-mediated growth and dispersion of Au nanoparticles in water via sequence engineering
dc.status.refereedYes
dc.date.Accepted2018-05-03
dc.date.application2018-05-03
dc.typeArticle
dc.type.versionAccepted manuscript
dc.identifier.doihttps://doi.org/10.1021/acs.jpcc.8b02392
dc.rights.licenseUnspecified
refterms.dateFOA2018-07-25T10:59:25Z
dc.openaccess.statusopenAccess


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