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    Structural Determinants of the Stability of Enzyme‐Responsive Polyion Complex Nanoparticles Targeting Pseudomonas aeruginosa’s Elastase

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    Insua_et_al_2018.pdf (4.371Mb)
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    Publication date
    2018-08
    Author
    Insua, I.
    Petit, M.
    Blackman, L.D.
    Keogh, R.
    Pitto-Barry, Anaïs
    O'Reilly, R.K.
    Peacock, F.A.
    Krachler, A.M.
    Fernandez-Trillo, F.
    Keyword
    Enzyme stability
    Polyion comple nanoparticles
    Degradable peptide
    Antimicrobial poly(ethylene imine)
    Rights
    (c) 2018 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Here, we report how the stability of polyion complex (PIC) particles containing Pseudomonas aeruginosa’s elastase (LasB) degradable peptides and antimicrobial poly(ethylene imine) is significantly improved by careful design of the peptide component. Three LasB‐degradable peptides are reported herein, all of them carrying the LasB‐degradable sequence −GLA− and for which the number of anionic amino acids and cysteine units per peptide were systematically varied. Our results suggest that while net charge and potential to cross‐link via disulfide bond formation do not have a predictable effect on the ability of LasB to degrade these peptides, a significant effect of these two parameters on particle preparation and stability is observed. A range of techniques has been used to characterize these new materials and demonstrates that increasing the charge and cross‐linking potential of the peptides results in PIC particles with better stability in physiological conditions and upon storage. These results highlight the importance of molecular design for the preparation of PIC particles and should underpin the future development of these materials for responsive drug delivery.
    URI
    http://hdl.handle.net/10454/15921
    Version
    Published version
    Citation
    Insua I, Petit M, Blackman LD et al (2018) Structural Determinants of the Stability of Enzyme‐Responsive Polyion Complex Nanoparticles Targeting Pseudomonas aeruginosa’s Elastase. ChemNanoMat. 4(8): 807-814.
    Link to publisher’s version
    https://doi.org/10.1002/cnma.201800054
    Type
    Article
    Collections
    Life Sciences Publications

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