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dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorAnderson, Diana
dc.date.accessioned2018-04-30T09:18:56Z
dc.date.available2018-04-30T09:18:56Z
dc.date.issued2016-10-27
dc.identifier.citationNajafzadeh M and Anderson D (2016) The use of isolated peripheral lymphocytes and human whole blood in the comet assay. Protocol Exchange. 5207.en_US
dc.identifier.urihttp://hdl.handle.net/10454/15701
dc.descriptionYesen_US
dc.description.abstractThe comet assay is a sensitive method used to detect DNA damage, measuring DNA breaks and alkali labile lesions in eukaryotic cells. Here, the use of whole blood in the alkaline gel electrophoresis method is described. Two hundred and seventy blood samples from individuals were examined: 120 healthy individuals, 65 suspected or pre-cancerous individuals and 85 cancer patients. Each sample was divided into two identical volumes in different falcon tubes. The blood was prepared and stored by adding the same amount of RPMI medium and 10% DMSO. Using the Student’s t-Test, the data showed a p value = 0.59 for Olive tail moment (OTM) and 0.16 for % tail DNA, and no statistically significant differences between the two methods, with or without treatment. In conclusion, using whole blood instead of isolated lymphocytes saves time, is still very sensitive and requires less than 20 µL of blood from each individual.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://www.nature.com/protocolexchange/protocols/5207en_US
dc.rights© 2016 Nature Publishing Group. This is an Open Access article distributed under the Creative Commons CC-BY-NC license (https://creativecommons.org/licenses/by-nc/3.0/igo/)
dc.subjectPeripheral lymphocytesen_US
dc.subjectWhole blooden_US
dc.subjectComet assayen_US
dc.titleThe use of isolated peripheral lymphocytes and human whole blood in the comet assayen_US
dc.status.refereedNoen_US
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-28T03:52:30Z


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