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dc.contributor.authorOsman, Ilham F.
dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorSharma, Vyom
dc.contributor.authorShukla, Ritesh K
dc.contributor.authorJacob, B.K.
dc.contributor.authorDhawan, A.
dc.contributor.authorAnderson, Diana
dc.date.accessioned2018-04-30T09:05:00Z
dc.date.available2018-04-30T09:05:00Z
dc.date.issued2018-01-01
dc.identifier.citationOsman IF, Najafzadeh M, Sharma V et al (2018) Journal of Nanotechnology and Nanomedicine. 18(1): 544-555.en_US
dc.identifier.urihttp://hdl.handle.net/10454/15700
dc.descriptionNoen_US
dc.description.abstractNanotechnology has preceded nanotoxicology and little is known of the effects of nanoparticles in human systems, let alone in diseased individuals. Therefore, the effects of titanium dioxide (TiO2) nanoparticles in peripheral blood lymphocytes from patients with respiratory diseases [lung cancer, chronic obstructive pulmonary disease (COPD) and asthma] were compared with those in healthy Individuals, to determine differences in sensitivity to nanochemical insult. The Comet assay was performed according to recommended guidelines. The micronucleus assay and ras oncoprotein detection were conducted according to published standard methods. The results showed statistically significant concentration-dependent genotoxic effects of TiO2 NPs in both respiratory patient and control groups in the Comet assay. The TiO2 NPs caused DNA damage in a concentration dependent manner in both groups (respiratory and healthy controls) with the exception of the lowest TiO2 concentration (10 µg/ml) which did not induce significant damage in healthy controls (ns). When OTM data were used to compare the whole patient group and the control group, the patient group had more DNA damage (p > 0.001) with the exception of 10 µg/ml of TiO2 that caused less significant damage to patient lymphocytes (p < 0.05). Similarly, there was an increase in the pattern of cytogenetic damage measured in the MN assay without statistical significance except when compared to the negative control of healthy individuals. Furthermore, when modulation of ras p21 expression was investigated, regardless of TiO2 treatment, only lung cancer and COPD patients expressed measurable ras p21 levels. All results were achieved in the absence of cytotoxicity.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1166/jnn.2018.15236en_US
dc.subjectTiO2 NPsen_US
dc.subjectDNA damageen_US
dc.subjectLymphocytesen_US
dc.subjectRespiratory diseasesen_US
dc.titleTiO2 NPs induce DNA damage in lymphocytes from healthy individuals and patients with respiratory diseases-An Ex vivo/In vitro Studyen_US
dc.status.refereedNoen_US
dc.typeArticleen_US
dc.type.versionNo full-text in the repositoryen_US


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