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    TiO2 NPs induce DNA damage in lymphocytes from healthy individuals and patients with respiratory diseases-An Ex vivo/In vitro Study

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    Publication date
    2018-01-01
    Author
    Osman, Ilham F.
    Najafzadeh, Mojgan
    Sharma, Vyom
    Shukla, Ritesh K
    Jacob, B.K.
    Dhawan, A.
    Anderson, Diana
    Keyword
    TiO2 NPs
    DNA damage
    Lymphocytes
    Respiratory diseases
    Peer-Reviewed
    No
    
    Metadata
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    Abstract
    Nanotechnology has preceded nanotoxicology and little is known of the effects of nanoparticles in human systems, let alone in diseased individuals. Therefore, the effects of titanium dioxide (TiO2) nanoparticles in peripheral blood lymphocytes from patients with respiratory diseases [lung cancer, chronic obstructive pulmonary disease (COPD) and asthma] were compared with those in healthy Individuals, to determine differences in sensitivity to nanochemical insult. The Comet assay was performed according to recommended guidelines. The micronucleus assay and ras oncoprotein detection were conducted according to published standard methods. The results showed statistically significant concentration-dependent genotoxic effects of TiO2 NPs in both respiratory patient and control groups in the Comet assay. The TiO2 NPs caused DNA damage in a concentration dependent manner in both groups (respiratory and healthy controls) with the exception of the lowest TiO2 concentration (10 µg/ml) which did not induce significant damage in healthy controls (ns). When OTM data were used to compare the whole patient group and the control group, the patient group had more DNA damage (p > 0.001) with the exception of 10 µg/ml of TiO2 that caused less significant damage to patient lymphocytes (p < 0.05). Similarly, there was an increase in the pattern of cytogenetic damage measured in the MN assay without statistical significance except when compared to the negative control of healthy individuals. Furthermore, when modulation of ras p21 expression was investigated, regardless of TiO2 treatment, only lung cancer and COPD patients expressed measurable ras p21 levels. All results were achieved in the absence of cytotoxicity.
    URI
    http://hdl.handle.net/10454/15700
    Version
    No full-text in the repository
    Citation
    Osman IF, Najafzadeh M, Sharma V et al (2018) Journal of Nanoscience and Nanotechnology. 18(1): 544-555.
    Link to publisher’s version
    https://doi.org/10.1166/jnn.2018.15236
    Type
    Article
    Collections
    Life Sciences Publications

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