Show simple item record

dc.contributor.authorHabas, Khaled S.A.*
dc.contributor.authorBrinkworth, Martin H.*
dc.contributor.authorAnderson, Diana*
dc.date.accessioned2018-04-26T14:24:20Z
dc.date.available2018-04-26T14:24:20Z
dc.date.issued2018-06
dc.identifier.citationHabas K, Brinkworth MH and Anderson D (2018) Silver nanoparticle-mediated cellular responses in isolated primary Sertoli cells in vitro. Food and Chemical Toxicology. 116(B): 182-188.en_US
dc.identifier.urihttp://hdl.handle.net/10454/15658
dc.descriptionYesen_US
dc.description.abstractThe present study explored the mechanism of cytotoxic and genotoxic effects of AgNPs on a primary culture of mouse Sertoli cells in vitro. To understand the possible molecular mechanisms of testicular lesions following exposure to AgNPs, isolated Sertoli cells were exposed to 5, 10, or 15 μg/ml. DNA damage in the Comet assay and apoptosis in the TUNEL assay were evaluated. The mRNA expression of p53 and bcl-2 genes and their proteins involved in apoptosis was also investigated. The antioxidant status of treated Sertoli cells was determined by measuring superoxide dismutase (SOD-1), catalase (CAT), and glutathione peroxidase (GPX-1) and superoxide dismutase (SOD-1) using quantitative polymerase chain reaction (qPCR)qPCR. The superoxide anions were detected using the nitroblue tetrazolium (NBT) reduction assay. Results indicated that AgNP exposure causes increased oxidative stress levels. The activation of p53, repression of bcl-2 and reduction of endogenous antioxidant enzymes were also involved in these mechanistic pathways, leading to reduced cell numbers and cell detachment.en_US
dc.description.sponsorshipThe Sponsorship of the Libyan Government of a PhD studentship to Khaled Habasen_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.fct.2018.04.030en_US
dc.rights© 2018 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en_US
dc.subjectSilver nanoparticles; Sertoli cell; DNA damage; Oxidative stress; Gene expression; Endogenous antioxidant enzymesen_US
dc.titleSilver nanoparticle-mediated cellular responses in isolated primary Sertoli cells in vitroen_US
dc.status.refereedYesen_US
dc.date.Accepted2018-04-13
dc.date.application2018-04-14
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2019-04-15T08:08:20Z


Item file(s)

Thumbnail
Name:
Manuscript.docx
Size:
1.544Mb
Format:
Unknown
Description:
To keep suppressed
Thumbnail
Name:
Habas_et_al_Food_&_Chemical_To ...
Size:
1.114Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record