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dc.contributor.authorChan, K.*
dc.contributor.authorPriya, K.*
dc.contributor.authorChang, Chien-Yi*
dc.contributor.authorAbdul Rahman, A.Y.*
dc.contributor.authorTee, K.K.*
dc.contributor.authorYin, W.*
dc.date.accessioned2018-03-12T14:58:47Z
dc.date.available2018-03-12T14:58:47Z
dc.date.issued2016-07-19
dc.identifier.citationChan K, Priya K, Chang C et al (2016) Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures. PeerJ. 4:e2223.en_US
dc.identifier.urihttp://hdl.handle.net/10454/15203
dc.descriptionYesen_US
dc.description.abstractFunctional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 C) and an arbitrarily-selected temperature of 46 C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.en_US
dc.description.sponsorshipUniversity of Malaya High Impact Research (HIR) UM-MOHE HIR Grants (UM.C/625/1/HIR/MOHE/CHAN/14/1, No. H-50001-A000027; UM.C/625/1/HIR/MOHE/CHAN/01, No. A000001-50001); PPP Grant (PG081-2015B)en_US
dc.language.isoenen_US
dc.rights© 2016 Chan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.en_US
dc.subjectHeat shock; RNA sequencing; Pseudomonas aeruginosa PAO1; Transcriptome; Gene expressionen_US
dc.titleTranscriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperaturesen_US
dc.status.refereedYesen_US
dc.date.Accepted2016-06-15
dc.date.application2016-07-19
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.7717/peerj.2223
refterms.dateFOA2018-07-28T03:21:33Z


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