Browsing Life Sciences by Author "Unciti-Broceta, J.D."
Tracking cell proliferation using a nanotechnology based approachAltea-Manzano, P.; Unciti-Broceta, J.D.; Cano-Cortes, V.; Ruiz-Blas, M.P.; Valero-Grinan, Teresa M.; Diaz-Mochon, J.J.; Sanchez-Martin, R. (2017-07)To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed.