• Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface

      Jamil, M. Mahadi Abdul; Zaltum, M.A.M.; Rahman, N.A.A.; Ambar, R.; Denyer, Morgan C.T.; Javed, F.; Sefat, Farshid; Mozafari, M.; Youseffi, Mansour (2018)
      Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM) molecules including collagen, fibronectin and laminin can be examined using micro contact printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration, differentiation and network formation in-vitro. This study investigates the effect of microcontact printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of 25μm, 50μm and 100μm width. However, HeLa cells seeded on 50μm induced the best alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50μm wide fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was influenced by 100μs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachment and cell guidance. Understanding this further may have applications in enhancing tissue graft generation and potentially wound repair.