• Evaluation of the toxicity of two electron-deficient half-sandwich complexes against human lymphocytes from healthy individuals

      Habas, Khaled S.A.; Soldevila Barreda, Joan J.; Azmanova, Maria; Rafols, Laia; Pitto-Barry, Anaïs; Anderson, Diana; Barry, Nicolas P.E. (2021-02)
      Electron‐deficient half‐sandwich complexes are a class of under‐studied organometallics with demonstrated potential as metallodrug candidates. The present study investigates the effect of two 16‐electron organoruthenium complexes ([( p‐ cym)Ru(benzene‐1,2‐dithiolato)] ( 1 ) and [( p ‐cym)Ru(maleonitriledithiolate)] ( 2 )) on the cell viability of non‐immortalised human lymphocytes from healthy individuals. The genotoxic effects of 1 and 2 in lymphocytes using the Comet and cytokinesis‐block micronucleus assays is also investigated. Gene expression studies were carried out on a panel of genes involved in apoptosis and DNA damage repair response. Results show that the two 16‐electron complexes do not have significant effect on the cell viability of human lymphocytes from healthy individuals. However, an increase in DNA damage is induced by both compounds, presumably through oxidative stress production.
    • Synthesis, characterisation, and in vitro anticancer activity of catalytically active indole-based half-sandwich complexes

      Soldevila-Barreda, Joan J.; Fawibe, K.B.; Azmanova, Maria; Rafols, Laia; Pitto-Barry, Anaïs; Eke, U.B.; Barry, Nicolas P.E. (2020-10-03)
      The synthesis, characterisation and evaluation of the in vitro cytotoxicity of four indole-based half-sandwich metal complexes towards two ovarian cancer cell lines (A2780 and A2780cisR) and one normal prostate cell line (PNT2) are presented herein. Although capable of inducing catalytic oxidation of NADH and able to reduce NAD+ with high turnover frequencies, in cells and in the presence of sodium formate, these complexes also strongly interact with biomolecules such as glutathione. This work highlights that efficient out-of-cells catalytic activity might lead to higher reactivity towards biomolecules, thus inhibiting the in-cells catalytic processes.