• Pandoraea sp. strain E26: discovery of its quorum-sensing properties via whole-genome sequence analysis

      Chan, K; Yin, W.; Tee, K.K.; Chang, Chien-Yi; Priya, K. (2015-05)
      We report the draft genome sequence of Pandoraea sp. strain E26 isolated from a former landfill site, sequenced by the Illumina MiSeq platform. This genome sequence will be useful to further understand the quorum-sensing system of this isolate.
    • Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures

      Chan, K.; Priya, K.; Chang, Chien-Yi; Abdul Rahman, A.Y.; Tee, K.K.; Yin, W. (2016-07-19)
      Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 C) and an arbitrarily-selected temperature of 46 C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.