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dc.contributor.authorForkuo, A.D.*
dc.contributor.authorAnsah, C.*
dc.contributor.authorMensah, K.B.*
dc.contributor.authorAnnan, K.*
dc.contributor.authorGyan, B.*
dc.contributor.authorTheron, A.*
dc.contributor.authorMancama, D.*
dc.contributor.authorWright, Colin W.*
dc.date.accessioned2018-01-16T10:28:03Z
dc.date.available2018-01-16T10:28:03Z
dc.date.issued2017-12
dc.identifier.citationForkuo AD, Ansah C, Mensah KB et al (2017) In vitro anti-malarial interaction and gametocytocidal activity of cryptolepine. Malaria Journal. 16: 496.en_US
dc.identifier.urihttp://hdl.handle.net/10454/14520
dc.descriptionYesen_US
dc.description.abstractBackground: Discovery of novel gametocytocidal molecules is a major pharmacological strategy in the elimination and eradication of malaria. The high patronage of the aqueous root extract of the popular West African anti-malarial plant Cryptolepis sanguinolenta (Periplocaceae) in traditional and hospital settings in Ghana has directed this study investigating the gametocytocidal activity of the plant and its major alkaloid, cryptolepine. This study also investigates the anti-malarial interaction of cryptolepine with standard anti-malarials, as the search for new anti-malarial combinations continues. Methods: The resazurin-based assay was employed in evaluating the gametocytocidal properties of C. sanguinolenta and cryptolepine against the late stage (IV/V) gametocytes of Plasmodium falciparum (NF54). A fixed ratio method based on the SYBR Green I fluorescence-based assay was used to build isobolograms from a combination of cryptolepine with four standard anti-malarial drugs in vitro using the chloroquine sensitive strain 3D7. Results: Cryptolepis sanguinolenta ( IC50 = 49.65 nM) and its major alkaloid, cryptolepine ( IC50 = 1965 nM), showed high inhibitory activity against the late stage gametocytes of P. falciparum (NF54). In the interaction assays in asexual stage, cryptolepine showed an additive effect with both lumefantrine and chloroquine with mean ΣFIC50s of 1.017 ± 0.06 and 1.465 ± 0.17, respectively. Cryptolepine combination with amodiaquine at therapeutically relevant concentration ratios showed a synergistic effect (mean ΣFIC50 = 0.287 ± 0.10) whereas an antagonistic activity (mean ΣFIC50 = 4.182 ± 0.99) was seen with mefloquine. Conclusions: The findings of this study shed light on the high gametocytocidal properties of C. sanguinolenta and cryptolepine attributing their potent anti-malarial activity mainly to their effect on both the sexual and asexual stages of the parasite. Amodiaquine is a potential drug partner for cryptolepine in the development of novel fixed dose combinations.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1186/s12936-017-2142-zen_US
dc.rights© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.subjectGametocytocidal; Malaria; Anti-malarial drug combinations; Cryptolepis sanguinolenta; Cryptolepineen_US
dc.titleIn vitro anti-malarial interaction and gametocytocidal activity of cryptolepineen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-12-18
dc.date.application2017-12-28
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-28T02:26:25Z


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