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    Structural properties governing drug-plasma protein binding determined by high-performance liquid chromatography method

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    Liu_et_al_JPBA.pdf (636.7Kb)
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    Publication date
    2018-02
    Author
    Kamble, Sharad R.
    Loadman, Paul M.
    Abraham, M.H.
    Liu, Xiangli
    Keyword
    Plasma proteins; Binding; HPLC; Retention factors (k); Linear free energy relationship; Abraham (Absolv) descriptors
    Rights
    © 2017 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
    Peer-Reviewed
    Yes
    
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    Abstract
    The high-performance liquid chromatography (HPLC) method employing stationary phases immobilized with plasma proteins was used for this study to investigate the structural properties governing drug-plasma protein binding. A set of 65 compounds with a broad range of structural diversity (in terms of volume, hydrogen-bonding, polarity and electrostatic force) were selected for this purpose. The Abraham linear free energy relationship (LFER) analyses of the retention factors on the immobilized HSA (human serum albumin) and AGP (α1-acid glycoprotein) stationary phases showed that McGowan’s characteristic molecular volume (V), dipolarity/polarizability (S) and hydrogen bond basicity (B) are the three significant molecular descriptors of solutes determining the interaction with immobilized plasma proteins, whereas excess molar refraction (E) is less important and hydrogen bond acidity (A) is not of statistical significance in both systems, for electrically neutral compounds. It was shown that ionised acids, as carboxylate anions, bind very strongly to the immobilized HSA stationary phase and that ionised bases, as cations bind strongly to the AGP stationary phase. This is the first time that the effect of ionised species on plasma protein binding has been determined quantitatively; the increased binding of acids to HSA is due almost entirely to acids in their ionised form.
    URI
    http://hdl.handle.net/10454/13844
    Version
    Accepted Manuscript
    Citation
    Kamble S, Loadman P, Abraham MH et al (2018) Structural properties governing drug-plasma protein binding determined by high-performance liquid chromatography method. Journal of Pharmaceutical and Biomedical Analysis. 149: 16-21.
    Link to publisher’s version
    https://doi.org/10.1016/j.jpba.2017.10.022
    Type
    Article
    Collections
    Life Sciences Publications

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