Tracking cell proliferation using a nanotechnology based approach
dc.contributor.author | Altea-Manzano, P. | * |
dc.contributor.author | Unciti-Broceta, J.D. | * |
dc.contributor.author | Cano-Cortes, V. | * |
dc.contributor.author | Ruiz-Blas, M.P. | * |
dc.contributor.author | Valero-Grinan, Teresa M. | * |
dc.contributor.author | Diaz-Mochon, J.J. | * |
dc.contributor.author | Sanchez-Martin, R. | * |
dc.date.accessioned | 2017-10-03T10:36:04Z | |
dc.date.available | 2017-10-03T10:36:04Z | |
dc.date.issued | 2017-07 | |
dc.identifier.citation | Altea-Manzano P, Unciti-Broceta JD, Cano-Cortes V, Ruiz-Blas MP, Valero-Grinan Teresa M, Diaz-Mochon JJ and Sanchez-Martin R (2017) Tracking cell proliferation using a nanotechnology-based approach. Nanomedicine. 12(13): 1591-1605. | en_US |
dc.identifier.uri | http://hdl.handle.net/10454/13342 | |
dc.description | yes | en_US |
dc.description.abstract | To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. | en_US |
dc.language.iso | en | en_US |
dc.rights | © 2017 IOP Publishing. Reproduced in accordance with the publisher's self-archiving policy. | en_US |
dc.subject | Cell proliferation; Cell tracking; Flow cytometry; Fluorescent nanoparticles; Nanofection | en_US |
dc.title | Tracking cell proliferation using a nanotechnology based approach | en_US |
dc.status.refereed | yes | en_US |
dc.date.application | 2017-05-17 | |
dc.type | Article | en_US |
dc.type.version | Accepted Manuscript | en_US |
dc.identifier.doi | https://doi.org/10.2217/nnm-2017-0118 | |
refterms.dateFOA | 2018-05-17T00:00:00Z |