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dc.contributor.authorAltea-Manzano, P.*
dc.contributor.authorUnciti-Broceta, J.D.*
dc.contributor.authorCano-Cortes, V.*
dc.contributor.authorRuiz-Blas, M.P.*
dc.contributor.authorValero-Grinan, Teresa M.*
dc.contributor.authorDiaz-Mochon, J.J.*
dc.contributor.authorSanchez-Martin, R.*
dc.date.accessioned2017-10-03T10:36:04Z
dc.date.available2017-10-03T10:36:04Z
dc.date.issued2017-07
dc.identifier.citationAltea-Manzano P, Unciti-Broceta JD, Cano-Cortes V, Ruiz-Blas MP, Valero-Grinan Teresa M, Diaz-Mochon JJ and Sanchez-Martin R (2017) Tracking cell proliferation using a nanotechnology-based approach. Nanomedicine. 12(13): 1591-1605.en_US
dc.identifier.urihttp://hdl.handle.net/10454/13342
dc.descriptionyesen_US
dc.description.abstractTo develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed.en_US
dc.language.isoenen_US
dc.rights© 2017 IOP Publishing. Reproduced in accordance with the publisher's self-archiving policy.en_US
dc.subjectCell proliferation; Cell tracking; Flow cytometry; Fluorescent nanoparticles; Nanofectionen_US
dc.titleTracking cell proliferation using a nanotechnology based approachen_US
dc.status.refereedyesen_US
dc.date.application2017-05-17
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
dc.identifier.doihttps://doi.org/10.2217/nnm-2017-0118
refterms.dateFOA2018-05-17T00:00:00Z


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