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dc.contributor.authorBerrout, J.*
dc.contributor.authorKyriakopoulou, E.*
dc.contributor.authorMoparthi, L.*
dc.contributor.authorHogea, A.S.*
dc.contributor.authorBerrout, L.*
dc.contributor.authorIvan, C.*
dc.contributor.authorLorger, M.*
dc.contributor.authorBoyle, J.*
dc.contributor.authorPeers, C.*
dc.contributor.authorMuench, S.*
dc.contributor.authorElies, Jacobo*
dc.contributor.authorHu, X.*
dc.contributor.authorHurst, C.*
dc.contributor.authorHall, T.*
dc.contributor.authorUmamaheswaran, S.*
dc.contributor.authorWesley, L.*
dc.contributor.authorGagea, M.*
dc.contributor.authorShires, M.*
dc.contributor.authorManfield, I.*
dc.contributor.authorKnowles, M.A.*
dc.contributor.authorDavies, S.*
dc.contributor.authorSuhling, K.*
dc.contributor.authorGonzalez, Y.T.*
dc.contributor.authorCarragher, N.*
dc.contributor.authorMacleod, K.*
dc.contributor.authorAbbott, N.J.*
dc.contributor.authorCalin, G.A.*
dc.contributor.authorGamper, N.*
dc.contributor.authorZygmunt, P.M.*
dc.contributor.authorTimsah, Z.*
dc.date.accessioned2017-08-23T11:42:52Z
dc.date.available2017-08-23T11:42:52Z
dc.date.issued2017-10
dc.identifier.citationBerrout J, Kyriakopoulou E, Moparthi L et al (2017) TRPA1-FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3p. Nature Communications. Accepted for Publication.en_US
dc.identifier.urihttp://hdl.handle.net/10454/12962
dc.descriptionYesen_US
dc.description.abstractRecent evidence suggests that the ion channel TRPA1 is implicated in lung adenocarcinoma (LUAD) where its role and mechanism of action remain unknown. We have previously established that the membrane receptor FGFR2 drives LUAD progression through aberrant protein-protein interactions mediated via its C-terminal proline rich motif. Here, we report that the N-terminal ankyrin repeats of TRPA1 directly bind to the C-terminal proline rich motif of FGFR2 inducing the constitutive activation of the receptor, thereby prompting LUAD progression and metastasis. Furthermore, we show that upon metastasis to the brain, TRPA1 gets depleted, an effect triggered by the transfer of TRPA1-targeting exosomal microRNA (miRNA-142-3p) from brain astrocytes to cancer cells. This downregulation, in turn, inhibits TRPA1-mediated activation of FGFR2 hindering the metastatic process. Our study reveals a direct binding event and characterizes the role of TRPA1 ankyrin repeats in regulating FGFR2-driven oncogenic process; a mechanism that is hindered by miRNA-142-3p.
dc.description.sponsorshipFaculty of Biological Sciences at the University of Leeds, Wellcome Trust Seed Award, Royal Society Research Grant RG150100, MR/K021303/1, Swedish Research Council (2014-3801) and the Medical Faculty at Lund University.en_US
dc.language.isoenen_US
dc.rights© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectIon channel TRPA1; Lung adenocarcinoma (LUAD); FGFR2; N-terminal ankyrin; Brain metastasesen_US
dc.titleTRPA1- FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3pen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-08-08
dc.date.application2017-10-16
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.1038/s41467-017-00983-w


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