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dc.contributor.authorZilic, L.
dc.contributor.authorWilshaw, Stacy-Paul
dc.contributor.authorHaycock, J.W.
dc.date.accessioned2017-08-22T15:48:26Z
dc.date.available2017-08-22T15:48:26Z
dc.date.issued2016-09
dc.identifier.citationZilic L, Wilshaw S-P and Haycock JW (2017) Decellularisation and histological characterisation of porcine peripheral nerves. Biotechnology and Bioengineering.113(9): 2041–2053.en_US
dc.identifier.urihttp://hdl.handle.net/10454/12941
dc.descriptionyesen_US
dc.description.abstractPeripheral nerve injuries affect a large proportion of the global population, often causing significant morbidity and loss of function. Current treatment strategies include the use of implantable nerve guide conduits (NGC's) to direct regenerating axons between the proximal and distal ends of the nerve gap. However, NGC's are limited in their effectiveness at promoting regeneration Current NGCs are not suitable as substrates for supporting either neuronal or Schwann cell growth, as they lack an architecture similar to that of the native extracellular matrix (ECM) of the nerve. The aim of this study was to create an acellular porcine peripheral nerve using a novel decellularisation protocol, in order to eliminate the immunogenic cellular components of the tissue, while preserving the three-dimensional histoarchitecture and ECM components. Porcine peripheral nerve (sciatic branches were decellularised using a low concentration (0.1%; w/v) sodium dodecyl sulphate in conjunction with hypotonic buffers and protease inhibitors, and then sterilised using 0.1% (v/v) peracetic acid. Quantitative and qualitative analysis revealed a ≥95% (w/w) reduction in DNA content as well as preservation of the nerve fascicles and connective tissue. Acellular nerves were shown to have retained key ECM components such as collagen, laminin and fibronectin. Slow strain rate to failure testing demonstrated the biomechanical properties of acellular nerves to be comparable to fresh controls. In conclusion, we report the production of a biocompatible, biomechanically functional acellular scaffold, which may have use in peripheral nerve repair.en_US
dc.description.sponsorshipEngineering and Physical Sciences Research Council. Grant Number: EPSRC EP/F500513/1en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1002/bit.25964en_US
dc.rights© 2016 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectNerve; Porcine peripheral nerve; Decelluarisation; Tissue engineering; Schwann cellen_US
dc.titleDecellularisation and histological characterisation of porcine peripheral nervesen_US
dc.status.refereedyesen_US
dc.date.Accepted2016-02-21
dc.date.application2016-03-30
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-26T08:54:59Z


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