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dc.contributor.authorDul, M.*
dc.contributor.authorPaluch, Krzysztof J.*
dc.contributor.authorHealy, A.M.*
dc.contributor.authorSasse, A.*
dc.contributor.authorTajber, L.*
dc.date.accessioned2017-06-27T09:13:34Z
dc.date.available2017-06-27T09:13:34Z
dc.date.issued2017-06
dc.identifier.citationDul M, Paluch KJ, Healy AM et al (2017) Optimisation of the self-assembly process: production of stable, alginate-based polyelectrolyte nanocomplexes with protamine. Journal of Nanoparticle Research. 19:221.en_US
dc.identifier.urihttp://hdl.handle.net/10454/12340
dc.descriptionYesen_US
dc.description.abstractThe aim of this work was to investigate the possibility of covalent cross-linker-free, polyelectrolyte complex formation at the nanoscale between alginic acid (as sodium alginate, ALG) and protamine (PROT). Optimisation of the self-assembly conditions was performed by varying the type of polymer used, pH of component solutions, mass mixing ratio of the components and the speed and order of component addition on the properties of complexes. Homogenous particles with nanometric sizes resulted when an aqueous dispersion of ALG was rapidly mixed with a solution of PROT. The polyelectrolyte complex between ALG and PROT was confirmed by infrared spectroscopy. To facilitate incorporation of drugs soluble at low pH, pH of ALG dispersion was decreased to 2; however, no nanoparticles (NPs) were formed upon complexation with PROT. Adjusting pH of PROT solution to 3 resulted in the formation of cationic or anionic NPs with a size range 70–300 nm. Colloidal stability of selected alginic acid low/PROT formulations was determined upon storage at room temperature and in liquid media at various pH. Physical stability of NPs correlated with the initial surface charge of particles and was time- and pH-dependent. Generally, better stability was observed for anionic NPs stored as native dispersions and in liquids covering a range of pH.en_US
dc.description.sponsorshipThis study was funded by Merrion Pharmaceuticals Ireland. This work was also supported by the Synthesis and Solid State Pharmaceutical Centre funded by the Science Foundation Ireland under grant number 12/RC/2275.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1007/s11051-017-3901-zen_US
dc.subjectAlginate; Protamine; Nanoparticles; Polyelectrolyte complex; Dynamic light scattering; Infrared spectroscopyen_US
dc.titleOptimisation of the self-assembly process: production of stable, alginate-based polyelectrolyte nanocomplexes with protamineen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-05-22
dc.date.application2017-06-17
dc.typeArticleen_US
dc.type.versionAccepted Manuscripten_US
refterms.dateFOA2018-07-27T01:29:09Z


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